基于花生NAM群体产量性状的QTL定位

ultivated peanut (Arachis hypogaea L.) is rich in nutritional value and widely cultivated, being one of the important oil crops. Due to the limited arable land resources, increasing the yield per unit area of peanut has always been the primary goal of peanut breeders. The 100-pod weight, 100-seed weight and shelling percentage are the direct determinants of the yield per plant of peanut. In this study, the NAM population constructed by our research group was used, and the QTL loci controlling the traits of the 100-pod weight, 100-seed weight and shelling percentage were co-located by combining genome-wide association analysis (GWAS) and linkage analysis. The results showed that the correlations of the three traits in the NAM population were consistent with those in the four RIL populations, and the two traits were highly significantly positively correlated. The broad-sense heritability ranged from 0.79 to 0.91. The effects of environment, genotype and the interaction between environment and genotype on the phenotypes of the three traits were all highly significant. Linkage analysis detected 11, 41 and 46 QTL intervals related to the 100-pod weight, 100-seed weight and shelling percentage in multiple environments, respectively, which were located on chromosomes 3, 7 and 15. GWAS identified 372, 226 and 36 SNP loci related to the three traits, respectively, all on chromosome 7. The co-location of linkage analysis and GWAS identified 16, 28 and 8 overlapping intervals for the three traits, respectively.?These overlapping intervals can provide a theoretical basis for subsequent fine localization and molecular marker-assisted breeding.????