RIP1表达量在L929细胞死亡方式调控中的作用研究

Receptor-interacting protein kinase 1 (RIP1) is a serine/threonine protein kinase abundantly expressed in intestinal epithelial cells. It participates in critical cellular processes including cell survival, apoptosis, and programmed necrosis, yet the relationship between RIP1 expression levels and specific cell death modalities remains unclear. This study investigates the role of RIP1 expression levels in regulating cell death modalities in L929 cells. We generated RIP1-knockdown L929 cell lines via lentiviral transduction. Using Incucyte live-cell imaging and analysis system, combined with propidium iodide (PI) staining, we explored the impact of varying RIP1 expression levels on L929 cell death. RIP1-knockdown L929 cells were treated with zVAD + Nec-1, and the effects on cell death modalities were comprehensively analyzed using Incucyte live-cell imaging and Western blotting. Our results demonstrated that RIP1-knockdown L929 cells exhibited increased cell death, while L929 cells with near-complete RIP1 ablation were non-viable. Furthermore, RIP1 knockdown induced a switch in cell death modality from necroptosis to alternative cell death pathways in L929 cells. These findings indicate that RIP1 plays a critical role in maintaining the survival of L929 cells, and alterations in its expression levels can modulate cell death modalities. This study provides a theoretical basis for the future development of small-molecule RIP1 inhibitors.