两个玉米连锁群体的生育酚含量QTL定位

o elucidate the genetic variation mechanisms of tocopherol in maize kernels, two maize linkage populations, CIMBL83/GEMS41 and TY1/GEMS41, were selected as genetic materials. Genetic linkage maps were constructed based on genome-wide markers, and tocopherol content in maize kernels was measured using high-performance liquid chromatography (HPLC) as phenotypic data for QTL mapping. The main findings are as follows: In the CIMBL83/GEMS41 and TY1/GEMS41 populations, 1,488 and 1,167 polymorphic SNP markers were screened respectively to construct genetic linkage maps, with average genetic distances between markers of 1.26 cM and 1.84 cM. Basic statistical analysis of tocopherol-related phenotypic traits in both populations revealed abundant phenotypic variation, with total tocopherol content ranging from 7.94-45.11 μg/g in CIMBL83/GEMS41 and 6.25-55.21 μg/g in TY1/GEMS41, along with correlations among traits. QTL analysis using composite interval mapping (CIM) identified 7 QTLs in the CIMBL83/GEMS41 population and 12 QTLs in the TY1/GEMS41 population, including 5 major QTLs (PVE > 10%). The application of these major QTLs in breeding programs can effectively enhance tocopherol content in maize kernels, providing a valuable reference for developing high-tocopherol maize germplasm.