eIF2α敲除小鼠模型的构建

Objective: To construct a conditional heterozygous knockout mouse model of the eukaryotic translation initiation factor 2 alpha subunit (eIF2α) gene using CRISPR/Cas9 gene editing technology, given that eIF2α is a core regulatory protein of the integrated stress response (ISR). This model will provide a crucial experimental foundation for investigating ISR\'s biological functions in vivo. Methods: We used CRISPR/Cas9 to generate mice with targeted knockout of eIF2α exon 3 and bred the offspring, with successful construction confirmed by PCR genotyping and sequencing. Stable inheritance of the knockout strain was verified through F1 germline maintenance. Conclusion: The successful establishment of this eIF2α knockout mouse model provides a reliable tool for elucidating eIF2α\'s physiological functions in vivo.