黑莓的降脂活性及其入血成分分析

Objective To investigate the lipid-lowering effects of blackberry juice and freeze-dried blackberry powder in obese mice and analyze the changes in their serum metabolites to explore the underlying mechanisms. Methods High-fat diet-induced obese mice were treated with blackberry juice and freeze-dried blackberry powder for 6 weeks. The effects of treatment on body weight, blood glucose, glucose tolerance, and liver pathology were assessed. Additionally, serum metabolites were analyzed using UPLC-Q TOF-MSE to identify active components and their potential lipid-lowering mechanisms. Results Blackberry intervention significantly reduced weight gain induced by a high-fat diet and improved glucose metabolism. The high-dose groups of both blackberry juice and freeze-dried blackberry powder showed significant improvements in body weight, blood glucose, and glucose tolerance. Component analysis revealed that freeze-dried blackberry powder contained various active compounds, including glycosides, amino acids, and flavonoids, whereas blackberry juice primarily contained sugars and amino acids. Serum metabolite analysis indicated that freeze-dried blackberry powder regulated lipid metabolism through multiple components, such as sugars, amino acids, and terpenes, exhibiting a strong lipid-lowering effect. Blackberry juice primarily exerted its lipid-lowering effect by improving glucose metabolism and antioxidant activity. Conclusion Blackberry has significant lipid-lowering and glucose metabolism-improving effects. Its complex active components show potential for the prevention and treatment of obesity and metabolic syndrome, providing a theoretical basis for its clinical application in related diseases.