多功能小檗碱/黄芩苷自组装药物的制备与表征

his study prepared berberine (BBR) and baicalin (BA) into BA-BBR nanoparticles (BA-BBR NPs) through a one-step self-assembly reaction. Subsequently, the nanoparticles were further modified using a metal-polyphenol network (MPN) formed by the coordination of caffeic acid and iron ions, successfully synthesizing novel BA/BBR@MPN nanoparticles (BA/BBR@MPN NPs). Characterization analysis revealed that the BA/BBR@MPN NPs exhibited a spherical morphology with an average particle size of 241.15 ± 26.52 nm. Under weakly acidic conditions, the MPN decomposes to release iron ions, which subsequently trigger a Fenton-like reaction, catalyzing hydrogen peroxide to generate hydroxyl radicals for chemodynamic therapy (CDT). The generated hydroxyl radicals ( OH) further deplete glutathione (GSH) levels. Photothermal performance experiments demonstrated that BA/BBR@MPN NPs exhibit concentration-dependent and power-dependent temperature elevation, maintaining excellent photothermal stability even after multiple laser irradiations, with a photothermal conversion efficiency of 26.4%. Cytotoxicity assays and in vitro hemolysis tests confirmed the excellent biocompatibility of BA/BBR@MPN NPs. In conclusion, this study successfully developed a multifunctional BA/BBR@MPN NPs platform capable of combined chemodynamic therapy (CDT) and photothermal therapy (PTT), providing novel insights for potential antibacterial or antitumor applications.