匍匐翦股颖悬浮细胞培养体系建立初探

reeping bentgrass is a perennial herbaceous plant belonging to the Agrostis genus of the Poaceae family. As a cool-season turfgrass, it exhibits excellent adaptability and high ornamental value. Establishing a genetic transformation system by inducing callus formation in creeping bentgrass is an important approach to obtaining genetically superior traits.Plant cell suspension culture technology involvePreStudy on establishing a suspension cell culture system for creeping bentgrassliminary study on establishing a suspension cell culture system for creeping bentgrasss cultivating plant cells and small cell aggregates in liquid media. Suspension-cultured plant cells are not constrained by external factors, exhibit relatively short doubling times, and can produce higher levels of secondary metabolites. In order to establish a good and stable creeping bentgrass suspension culture system. The preliminary process about creeping bentgrass suspension cell\'s growth and proliferation was explored. This study provides theoretical support for the establishment of suspension system and genetic transformation system with strong viability and vigorous cell proliferation. The results showed that after subculture of callus for two times before the induction of suspension cells, the granular embryogenic callus with bright yellow color, loose texture, easy dispersion and hard to secret mucus could be obtained. At the initial stage of suspension culture, the subculture medium was changed every 3 days, and the fresh culture medium and the original culture medium were controlled at about 2:1, which helped to maintain the vigorous proliferation of cells. One month later, after the establishment of suspension system, the optimal subculture time of suspension cells in stable state was 8-10d. The ratio of fresh culture medium to original culture medium continued to be controlled at about 2:1, which was suitable for the regeneration of suspension cells of creeping bentgrass. The optimum pH value for the growth of suspension cells of the creeping bentgrass was between 5.0 and 5.5. 15 mg/L was suitable for Agrobacterium-mediated genetic transformation in the suspension cells of the creeping bentgrass.