陆地棉苗期基因GhirD11G005320的耐盐性鉴定

Soil salinization ranks among the crucial abiotic stresses, posing a substantial threat to agricultural production and ecological security. In saline - alkali soils, the excessive accumulation of soluble salts exerts a profound impact on the growth and development of the majority of plant species. Cotton, an important economic crop, demonstrates the ability to adapt to relatively adverse growth conditions and is thus hailed as a pioneer crop for cultivation in saline - alkali lands. Nevertheless, the research on the salt - tolerance mechanisms of cotton has advanced at a sluggish pace, necessitating further in - depth investigations.Notably, the salt - tolerance capacity of cotton varies markedly across different growth stages. Among these, the seedling stage is particularly sensitive to salt stress. In previous research, our research team identified a gene associated with salt tolerance, designated as GhirD11G005320.In the present study, a virus - induced gene silencing (VIGS) experiment was conducted on this gene. Additionally, the content of malondialdehyde (MDA) in the experimental materials was measured. The results indicated that following treatment with a 150 mmol/L NaCl solution for 5 hours, the seedlings in which GhirD11G005320 was silenced exhibited less bending of the young stems compared to the control group. After 5 days of treatment, the MDA content in the silenced group was significantly lower than that in the control group. These results preliminarily suggest that GhirD11G005320 negatively regulates the salt - tolerance response during the cotton seedling stage.