棉花泛素结合酶基因GhRCE1调控黄萎病抗性的功能研究

Objective] Through the identification of the disease resistance phenotype and the analysis of the molecular mechanism of the GhRCE1 gene in upland cotton, it was verified whether GhRCE1 is involved in regulating the resistance to Verticillium Wilt disease, and its mechanism of action was deeply analyzed. [Method] In this study, genetic, molecular biological and other means were employed to conduct functional identification on whether GhRCE1 regulates Verticillium Wilt resistance. By measuring the relevant indicators of reactive oxygen species and lignin content, the mechanism and molecular mechanism of GhRCE1 regulating Verticillium Wilt resistance in cotton were preliminarily revealed. [Result] GhRCE1 was induced to be expressed by the Verticillium Wilt fungus. In both the disease-resistant variety ND601 and the susceptible variety CCRI8, GhRCE1 was upregulated by the Verticillium Wilt fungus, but the response of GhRCE1 in ND601 was faster. GhRCE1 positively regulates the resistance to yellow-wilt in plants. Silencing leads to a significant decrease in resistance. Overexpression of GhRCE1 in Arabidopsis thaliana can significantly improve the resistance to yellow-wilt. GhRCE1 can positively regulate the resistance of cotton Verticillium Wilt disease by influencing the balance of reactive oxygen species and the content of lignin. [Conclusion] Molecular experimental methods confirmed that GhRCE1 positively regulates the resistance of plant Verticillium Wilt disease, providing a new perspective for the analysis of the disease resistance mechanism of cotton.