棉花抗黄萎病相关基因WRKY03的克隆和抗黄萎功能研究

otton is a crucial economic crop and raw material for the textile industry in China, playing a vital role in agricultural production. Verticillium wilt is a significant disease that severely impacts cotton yield and quality. WRKY transcription factors, one of the largest transcription factor families in plants, play an essential role in balancing plant growth and defense responses under stress conditions. In this study, we conducted a bioinformatics analysis of the cotton WRKY03 transcription factor. The gene is localized in the nucleus, with a CDS sequence of 1,724 bp in length, encoding a protein consisting of 573 amino acids and a molecular weight of 62.77 kDa. Using RT-PCR technoCloinCloing and Bioinformatics Analysis of the Verticillium wilt Resistance Gene WRKY03 and Bioinformatics Analysis of the Verticillium wilt Resistance Gene WRKY03 logy, we further investigated the expression patterns of WRKY03 in Gossypium barbadense (Pima90) and Gossypium hirsutum (NDM8). VIGS test showed that the gene negatively regulated verticillium wilt resistance response of cotton and its expression level was different in different parts of cotton.