大豆小热激蛋白GmHSP17.9功能鉴定

Soybean growth requires large amount of phosphorus, and low phosphorus stress seriously affects the yield and quality of soybean. Small heat shock proteins (sHSPs) are involved in regulating the growth and development of soybean and other plants under multiple abiotic stress, but whether they respond to low phosphorus stress has not been reported. Therefore, in this study, the expression characteristics of small heat shock protein GmHSP17.9 and the low phosphorus tolerance of gene-editing hsp17.9 mutant were identified. The results showed that the expressiFunctional analysis of small heat shock protein GmHSP17.9 in response to low phosphorus stress in soybeanon of GmHSP17.9 was increased by low phosphorus stress confirmed by qRT-PCR and promoter-GUS transgenic complex plants. In situ hybridization showed that GmHSP17.9 was specifically expressed in root vascular bundle tissues. Under low phosphorus condition, the growth of hsp17.9 mutant was inhibited, and its fresh and dry weight in ground, fresh and dry weight in underground and phosphorus content were significantly lower than those of wild type (WT). The allelic variation of exon of GmHSP17.9 can divide the natural population into two haplotypes, and the above-ground dry weight of the two haplotypes is significantly different under low phosphorus stress. This study preliminarily confirmed the low phosphorus tolerance function of small heat shock protein GmHSP17.9, which laid a foundation for further analysis of GmHSP17.9 regulation and mechanism of low phosphorus stress in soybean.