使用面筋聚集仪挖掘小麦面筋强度主效稳定QTL的研究

Gluten strength is a critical determinant of wheat flour processing quality; however, conventional farinograph-based methods exhibit poor reproducibility and high sample consumption. In this study, we employed an optimized GlutoPeak detection system combined with multi-environment phenotypic data from a recombinant inbred line (RIL) population (G8901 × PB3228) to conduct QTL mapping and genetic analysis of gluten strength. Key findings revealed: Strong positive correlations between gluten aggregation parameters (PMT, AM) and traditional mixograph peak time (r=0.83,p<0.01); High environmental stability of critical indicators including PMT and BEM, with coefficients of variation (CV) <5% across environments. Multi-environment QTL co-localization identified two major loci, qPMT.hbau-1D.1 (explaining 40.48% phenotypic variance in peak time) and qPM.hbau-1A.2 (9.89% variance in post-peak value), which colocalized with candidate genes encoding Glu-1Dx/Dy subunits, peroxidases, and glucosyltransferases. The standardized GlutoPeak workflow enabled precise dissection of gluten strength genetics, providing molecular markers and theoretical insights for wheat quality improvement.