基于CD30纳米抗体的CAR-T细胞对间变性大细胞淋巴瘤的抗肿瘤作用研究
Research on the Anti-Tumor Effects of CD30 Nanobody-Based CAR-T Cells in Anaplastic Large Cell Lymphoma
顾宇蓝 1游凤涛 2安钢力 1杨林3
作者信息
- 1. 苏州大学唐仲英血液学研究中心，苏州 215123
- 2. 博生吉医药科技（苏州）有限公司，江苏苏州 215123
- 3. 苏州大学唐仲英血液学研究中心，苏州 215123;博生吉医药科技（苏州）有限公司，江苏苏州 215123
- 折叠
摘要
目的：针对CD30阳性肿瘤，开发靶向CD30的嵌合抗原受体T细胞（Chimeric antigen receptor modified T cells, CAR-T），体外探究三种CAR-T细胞对于CD30阳性肿瘤的抗肿瘤疗效。方法：通过慢病毒转染的方式构建三种CD30-CAR-T，通过流式细胞术对慢病毒转导效率、体外杀伤能力、分化表型、表面活化及耗竭表达情况等进行检测。结果：成功制备了滴度较高的慢病毒，随后成功通过慢病毒转导的方式构建形成CD30-CAR-T细胞，最终验证了其在体外展示出较好的控制CD30阳性肿瘤生长的能力。4.结论：在本研究中成功构建了CD30-CAR-T细胞，对其在体外的抗肿瘤作用进行验证，具有较好的控制CD30阳性肿瘤进展的能力，为其临床治疗提供了一个新的方向。Abstract
Objective:To develop CD30-targeting chimeric antigen receptor-modified T cells (CAR-T cells) for CD30-positive tumors and evaluate the antitumor efficacy of three CAR-T cell types against CD30-positive tumors in vitro.Methods:Three CD30-CAR-T cell types were constructed via lentiviral transduction. Flow cytometry was used to assess lentiviral transduction efficiency,in vitro cytotoxic activity, differentiation phenotypes, and surface activation/exhaustion markers.Results:High-titer lentiviruses were successfully prepared, and CD30-CAR-T cells were generated via lentiviral transduction. These cells demonstrated robust ability to control the growth of CD30-positive tumors in vitro.Conclusion: This study successfully constructed CD30-CAR-T cells and validated theirin vitroantitumor effects against CD30-positive tumors, demonstrating promising potential for clinical translation. 关键词
肿瘤学/CD30/CAR-TKey words
Oncology/CD30/CAR-T引用本文复制引用
顾宇蓝,游凤涛,安钢力,杨林.基于CD30纳米抗体的CAR-T细胞对间变性大细胞淋巴瘤的抗肿瘤作用研究[EB/OL].(2025-06-17)[2025-12-14].http://www.paper.edu.cn/releasepaper/content/202506-75.学科分类
肿瘤学
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