蛋白结合适配体稳定无poly(A)的mRNA激活翻译

Objective: To explore the method of stable poly (A) -tailed RNA. Methods: Construct RNA-binding protein systems with potential stabilizing effects, such as LicT-RAT and MCP-MS2 systems. Simulate natural poly (A) -free RNA by resecting the poly (A) of the target gene, and study the stabilizing and activating effects of these two systems on RNA. Result: Both of the two constructed protein-binding aptamer systems can well stabilize poly (A) tail-free mRNA and promote the translation and expression of target genes. Conclusion: In this paper, two stable RNA systems, LicT-RAT and MCP-MS2, were successfully constructed, which have good application potential.