In vivo imaging of central nervous system fluid spaces using synchrotron radiation-based micro computed tomography
In vivo imaging of central nervous system fluid spaces using synchrotron radiation-based micro computed tomography
Current approaches to in vivo imaging of the mouse central nervous system (CNS) do not offer a combination of micrometer resolution and a whole-brain field of view. To address this limitation, we introduce an approach based on synchrotron radiation-based hard X-ray micro computed tomography (SR$μ$CT). We performed intravital SR$μ$CT acquisitions of mouse CNS fluid spaces at three synchrotron radiation facilities. Imaging was conducted on both anesthetized free-breathing and ventilated animals, with and without retrospective cardiac gating. We achieved whole-brain imaging at 6.3 $μ$m uniform voxel size, observed the distribution of cerebrospinal fluid (CSF) contrast agent over time and quantified choroid plexus movement. SR$μ$CT bridges the gap between multiphoton microscopy and magnetic resonance imaging, offering dynamic imaging with micrometer-scale resolution and whole-organ field of view. Intravital SR$μ$CT will play a crucial role in validating and integrating hypotheses on CSF dynamics and solute transport by providing unique data that cannot be acquired otherwise.
Marta Girona Alarcón、Willy Kuo、Mattia Humbel、Christine Tanner、Luca Fardin、Britta Bausch、Yann Decker、Irene Spera、Griffin Rodgers、Hans Deyhle、Alberto Bravin、Masato Hoshino、Arash Panahifar、Kentaro Uesugi、Sergei Gasilov、Petr Pleskač、Yuansheng Zhang、Diane de Zélicourt、Amandine Brenna、Ahmad Kamal Hamid、Pooya Razzaghi Khamesi、Britta Engelhardt、Steven T. Proulx、Bert Müller、Vartan Kurtcuoglu
医学研究方法神经病学、精神病学
Marta Girona Alarcón,Willy Kuo,Mattia Humbel,Christine Tanner,Luca Fardin,Britta Bausch,Yann Decker,Irene Spera,Griffin Rodgers,Hans Deyhle,Alberto Bravin,Masato Hoshino,Arash Panahifar,Kentaro Uesugi,Sergei Gasilov,Petr Pleskač,Yuansheng Zhang,Diane de Zélicourt,Amandine Brenna,Ahmad Kamal Hamid,Pooya Razzaghi Khamesi,Britta Engelhardt,Steven T. Proulx,Bert Müller,Vartan Kurtcuoglu.In vivo imaging of central nervous system fluid spaces using synchrotron radiation-based micro computed tomography[EB/OL].(2025-07-03)[2025-07-23].https://arxiv.org/abs/2507.03186.点此复制
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