Mis13磷酸化促进减数分裂中动粒的组装
Mis13 Phosphorylation promotes meiotic assembly of kinetochores
动粒在进入减数分裂前期时发生解体，并在减数分裂I期之前重新组装。这一过程的调控机制及其对减数分裂的影响还不清楚。本研究发现，在裂殖酵母中Aurora B激酶介导动粒蛋白Mis13的磷酸化，从而调控减数分裂中动粒的组装。在减数分裂前期，随着动粒的解体，细胞中观测不到Mis13-GFP荧光信号点；但在Mis13模拟磷酸化突变体中，能够观察到 Mis13-GFP荧光信号点，动粒蛋白Mis12和Nnf1在着丝粒上的定位明显增强。将细胞阻滞在减数分裂II的前期时，在Mis13模拟磷酸化突变体中，着丝粒上Mis13-GFP的荧光强度明显增强；在Mis13去磷酸化突变体中，Mis13-GFP的荧光强度明显降低。这些结果说明，Mis13磷酸化促进减数分裂中动粒的组装。
细胞生物学
减数分裂动粒显微技术动粒解体与组装裂殖酵母?
胡伟,刘影,孙力,董国琛,渡边嘉典,马玲玲,侯海彤.Mis13磷酸化促进减数分裂中动粒的组装[EB/OL].(2025-09-12)[2025-09-18].http://www.paper.edu.cn/releasepaper/content/202509-14.点此复制
Kinetochore disassembles during the prophase of meiosis and reassembles before meiosis I. The regulatory mechanism of this process and its impact on meiosis remain unclear. We find that Aurora B kinase mediates the phosphorylation of Mis13, thereby regulating the assembly of kinetochore during meiosis in fission yeast. During prophase of meiosis, Mis13-GFP foci largely disappear along with kinetochore disasseMis13 Phosphorylation promotes meiotic assembly of kinetochoresmbly; however, in the Mis13 phosphomimetic mutant, Mis13-GFP foci can be observed, and the centromeric localization of kinetochore proteins Mis12 and Nnf1 is significantly enhanced. When cells are arrested at the prophase of meiosis II, in the Mis13 phosphomimetic mutant, the intensity of Mis13-GFP foci is significantly enhanced; while in the Mis13 non-phosphorylatable mutant, the intensity of Mis13-GFP is significantly reduced. These results indicate that Mis13 phosphorylation promotes the kinetochore assembly during meiosis.
MeiosisKinetochoreMicroscopyKinetochore disassembly and assemblySchizosaccharomyces pombe
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