小麦转录因子基因 TabZIP23-6D 分子特征及抗旱功能研究
Molecular Characterization and Drought Resistance Function of Wheat Transcription Factor Gene TabZIP23-6D
刘金枝 1郭程瑾 1肖凯1
作者信息
- 1. 河北农业大学农学院，保定市 071000
- 折叠
摘要
碱性亮氨酸拉链（bZIP）转录因子在介导植物响应生物和非生物胁迫过程中发挥重要作用。本研究以通过转录组分析获得1个对干旱逆境产生明显应答的bZIP转录因子基因TabZIP23-6D为对象，对其调控小麦抗旱能力进行了鉴定。结果表明，TabZIP23-6D蛋白含有bZIP转录因子具有的典型的亮氨酸拉链二聚化区和多个DNA结合位点。TabZIP23-6D在小麦根叶组织中呈干旱诱导表达模式。基因转化分析表明，与野生型（WT）相比，干旱胁迫下过表达TabZIP23-6D株系生物量增加。表明供试基因在小麦干旱抵御过程中发挥正向调控作用。此外，供试基因过表达株系干旱处理下光合性能改善，气孔关闭速率加快，叶片失水率降低，细胞渗透物质可溶性糖、可溶性蛋白和脯氨酸含量显著增加，抗氧化酶SOD、POD和CAT活性增强。而敲除供试基因株系干旱处理下植株干质量和上述生理生化指标与过表达株系相比均呈相反趋势。本研究表明，TabZIP23-6D通过协同调控植物多个渗透胁迫生理生化过程，增强植株抗旱能力。该基因可作为小麦抗旱资源鉴定和节水栽培的重要分子指标。
Abstract
The basic leucine zipper (bZIP) transcription factors play a critical role in mediating plant responses to biotic and abiotic stresses. In this study, we investigated the function of TabZIP23-6D, a bZIP transcription factor gene in wheat, which was identified through transcriptome analysis as significantly responsive to drought stress. The results showed that the TabZIP23-6D protein contains a typical leucine zipper dimerization domain and multiple DNA-binding sites characteristic of bZIP transcription factors. Expression profiling showed that TabZIP23-6D was drought-inducible in both root and leaf tissues of wheat. Gene transformation analysis showed that overexpression of TabZIP23-6D led to increased biomass compared with the wild type (WT), indicating its positive regulatory role in drought tolerance. Furthermore, the overexpression lines exhibited improved photosynthetic performance, accelerated stomatal closure, reduced leaf water loss, and significant increases in soluble sugars, soluble proteins, and proline contents as osmotic substances, along with enhanced activities of antioxidant enzymes SOD, POD, and CAT. In contrast, the knockout lines showed opposite trends in plant dry weight and the above-mentioned physiological and biochemical indicators under drought stress compared with the overexpression lines. This study demonstrates that TabZIP23-6D enhances plant drought resistance by coordinately regulating multiple physiological and biochemical processes under osmotic stress. Therefore, TabZIP23-6D could serve as a valuable molecular marker for identifying drought-resistant wheat germplasms and water-saving cultivation.关键词
小麦/转录因子/干旱胁迫/基因功能/生理机制Key words
Wheat/Transcription factor/Drought stress/Gene function/physiological mechanism引用本文复制引用
刘金枝,郭程瑾,肖凯.小麦转录因子基因 TabZIP23-6D 分子特征及抗旱功能研究[EB/OL].(2025-12-19)[2026-04-03].http://www.paper.edu.cn/releasepaper/content/202512-32.学科分类
农业科学研究/分子生物学/植物学
评论