不同糖化血红蛋白水平患者尿蛋白质组修饰分析
Analysis of Urinary Proteome Modifications in Patients with Different Glycated Hemoglobin A1c Levels
陈昱臻 1高友鹤1
作者信息
- 1. 北京师范大学 生命科学学院 基因工程药物及生物技术北京市重点实验室
- 折叠
摘要
糖尿病作为全球重大公共卫生问题，早期诊断与及时干预至关重要。糖化血红蛋白是血糖管理的生物标志物，其水平与糖尿病发病风险呈连续性关联。本研究对比分析了两组不同糖化血红蛋白水平患者（[6.4±0.7]%和[8.6±1.6]%）与健康对照组的尿蛋白质组修饰，分别鉴定到1954种和5545种差异修饰肽段，两组中，呈现从有到无或从无到有变化的差异修饰肽段占比分别为48.8%、86.5%，同时，随机分组检验结果显示，两组中至少90.6%及94.1%的差异修饰肽段不是随机产生的。综上，尿蛋白质组修饰可以全面、系统地反映糖化血红蛋白水平升高的变化，且不同糖化血红蛋白水平对应不同的修饰组合，提示尿蛋白质组修饰具有反映糖化血红蛋白水平的应用潜力，为糖尿病早期诊断的研究打开新的窗口。
Abstract
Diabetes, a major global public health concern, requires early diagnosis and timely intervention. Glycated hemoglobin A1c (HbA1c) serves as a biomarker of glycemic management, with its levels showing a continuous relationship with the risk of developing diabetes. In this study, urinary proteome modifications were compared between each of the two patient groups with different HbA1c levels ([6.40.7]% and [8.61.6]%) and healthy controls. A total of 1,954 and 5,545 differentially modified peptides were identified in the two groups, respectively. Within each group, differentially modified peptides exhibiting changes from presence to absence or vice versa accounted for 48.8% and 86.5%, respectively. Additionally, results from the randomized grouping test indicated that at least 90.6% and 94.1% of these differentially modified peptides in each group were not randomly generated. In conclusion, urinary proteome modifications comprehensively and systematically reflect changes associated with elevated HbA1c levels, with distinct modification profiles corresponding to different HbA1c levels. These findings suggest that urinary proteome modifications have the potential to reflect HbA1c levels and offer a new perspective for research on the early diagnosis of diabetes.关键词
尿液/蛋白质组学/修饰/糖化血红蛋白/糖尿病/生物标志物Key words
urine/proteomics/modifications/glycated hemoglobin A1c/diabetes mellitus/biomarker引用本文复制引用
陈昱臻,高友鹤.不同糖化血红蛋白水平患者尿蛋白质组修饰分析[EB/OL].(2025-12-31)[2026-01-02].https://chinaxiv.org/abs/202512.00065.学科分类
临床医学/基础医学/医学研究方法
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