水稻谷胱甘肽还原酶OsGR2基因在盐胁迫响应中的功能初探
Preliminary Study on the Function of Rice Glutathione Reductase Gene OsGR2 in Salt Stress Response?
胡胜男 1王艳 1刘聪 2林建中 1刘选明2
作者信息
- 1. 湖南大学生物学院/隆平农学院，长沙，410000
- 2. 岳麓山实验室，长沙 410000
- 折叠
摘要
谷胱甘肽还原酶（glutathione reductase, GR）是植物抗氧化系统关键酶，但水稻中GR家族成员OsGR2在盐胁迫响应中的功能尚不明确。本研究利用CRISPR/Cas9和过表达技术构建了OsGR2转基因水稻材料，并对其耐盐性进行了鉴定。结果表明，过表达OsGR2显著增强水稻幼苗的耐盐性，而osgr2突变体对盐胁迫更为敏感。生理测定结果显示，盐胁迫下过表达株系中GR活性及还原型谷胱甘肽（GSH）含量均显著提高，丙二醛（MDA）含量和相对电导率则显著降低，而osgr2突变体则呈现相反趋势。综上，OsGR2通过维持GR活性正调控水稻苗期的耐盐性，其功能缺失则削弱抗氧化防御系统，加剧氧化损伤，使水稻对盐胁迫更加敏感。本研究为深入解析水稻抗氧化机制及耐盐遗传改良提供了理论依据和重要种质资源。
Abstract
Glutathione reductase (GR) is a key enzyme in the plant antioxidant system; however, the function of OsGR2, a member of the GR family in rice, in salt stress response remains unclear. In this study, OsGR2 transgenic rice lines were generated using CRISPR/Cas9 and overexpression techniques, and their salt tolerance was characterized. The results showed that overexpression of OsGR2 significantly enhanced salt tolerance in rice seedlings, whereas osgr2 mutants exhibited increased sensitivity to salt stress. Physiological assays revealed that under salt stress, the overexpression lines displayed significantly increased GR activity and reduced glutathione (GSH) content, along with significantly decreased malondialdehyde (MDA) content and relative electrical conductivity; in contrast, the osgr2 mutants showed opposite trends. Collectively, OsGR2 positively regulates salt tolerance at the seedling stage by maintaining GR activity, whereas loss of OsGR2 function weakens the antioxidant defense system, exacerbates oxidative damage, and renders rice more sensitive to salt stress. This study provides a theoretical basis and valuable germplasm resources for further exploration of the antioxidant mechanisms and genetic improvement of salt tolerance in rice.?关键词
生物学/水稻/OsGR2/盐胁迫/谷胱甘肽还原酶/抗氧化系统Key words
Biology/Rice/OsGR2/salt stress/glutathione reductase/antioxidant system引用本文复制引用
胡胜男,王艳,刘聪,林建中,刘选明.水稻谷胱甘肽还原酶OsGR2基因在盐胁迫响应中的功能初探[EB/OL].(2026-03-31)[2026-04-03].http://www.paper.edu.cn/releasepaper/content/202603-305.学科分类
农作物/植物保护/生物化学
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