花生AhFLA4基因克隆及响应旱盐胁迫的表达分析
Cloning of the Peanut AhFLA4 Gene and Functional Study of Its Response to Drought and Salt Stress
高健 1司爽 1赵佳慧 1杨鑫雷1
作者信息
- 1. 河北农业大学农学院，保定，071000
- 折叠
摘要
【目的】为探究AhFLA4基因序列特征及在两种株型花生品种的组织器官中干旱和盐胁迫下的表达模式。【方法】采用单基因生物信息学分析、基因克隆、亚细胞定位、花生表达特征分析，初步明确AhFLA4基因的表达模式。【结果】AhFLA4基因序列全长为858bp，编码285个氨基酸，蛋白质分子质量为30.15kD；AhFLA4基因编码的蛋白其二结构氨基酸序列α螺旋占26.67%，无规则卷曲占60%，延伸链占13.33%；信号肽分析和跨膜结构域分析结果显示，AhFLA4存在信号肽的概率为0.39在氨基酸序列的7-29处有跨膜螺旋，亚细胞定位预测该基因定位于细胞膜中；表达量分析表明，直立型花生JH5和匍匐型花生YUS1在不同组织中均呈现显著或极显著表达差异；盐胁迫和干旱胁迫下，在花生叶和根中，AhFLA4基因在YUS1与JH5中的表达均表现为"先升高-后降低-再升高"的趋势。【结论】AhFLA4基因能够响应干旱和盐胁迫，是花生潜在抗旱耐盐功能基因。
Abstract
[Purpose]To investigate the sequence characteristics of the AhFLA4 gene and its expression patterns in tissues and organs of two peanut cultivars under drought and salt stress. [Methods]Single-gene bioinformatics analysis, gene cloning, subcellular localization, and peanut expression profiling were employed to preliminarily elucidate the expression pattern of the AhFLA4 gene. [Results]The gene sequence has a full length of 858 bp, encoding 285 amino acids with a protein molecular weight of 30.15 kD. The secondary structure analysis of the AhFLA4-encoded protein revealed that α-helices account for 26.67%, random coils for 60%, and extended chains for 13.33%. Signal peptide analysis and transmembrane domain analysis indicated that AhFLA4 contains a signal peptide probability of 0.39, with transmembrane helices present at positions 7-29 of the amino acid sequence. Subcellular localization predictions suggest the gene is localized in the cell membrane. Expression analysis demonstrated significant or highly significant expression differences between JH5 and creeping peanut YUS1 across various tissues. Under salt and drought stress, AhFLA4 gene expression in peanut leaves and roots exhibited a "first increase, then decrease, followed by increase" trend in both YUS1 and JH5. [Conclusion]The AhFLA4 gene responds to salt stress and represents a potential salt-resistant functional gene in peanuts.关键词
花生/AhFLA4/基因克隆/表达分析/生物信息学Key words
Peanut/AhFLA4/Bioinformatics/Gene cloning/Expression analysis引用本文复制引用
高健,司爽,赵佳慧,杨鑫雷.花生AhFLA4基因克隆及响应旱盐胁迫的表达分析[EB/OL].(2026-04-21)[2026-04-22].http://www.paper.edu.cn/releasepaper/content/202604-155.学科分类
生物科学研究方法、生物科学研究技术/分子生物学
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