基于BSA-seq的花生种子大小物理性状定位
QTL mapping of physical traits related to peanut seed size based on BSA-seq
任常师 1李泽仁 1李松原 1李楚珩 1王嘉宝 1杨鑫雷1
作者信息
- 1. 河北农业大学农学院，保定，071000
- 折叠
摘要
【目的】本研究旨在挖掘与花生种子大小物理性状紧密相关的遗传位点，为高产分子育种提供理论依据与基因资源。【方法】以大粒地方种"秦皇岛立秧"和小粒地方种"平南石腰豆"为亲本构建的204个家系CSSL群体为研究材料，构建大小粒混池，通过BSA-seq测序对花生种子大小物理性状进行定位，并对候选区域进行基因注释。【结果】双亲及混池共检测到713,242个SNP和218,258个InDel；以95%置信水平筛选获得4个与种子大小物理性状相关的QTL区域（A05、A08、B05、B06）；以99%置信水平在A05染色体102.52-110.54 Mb（8.02 Mb）与B06染色体140.10 Mb~143.84 Mb（3.74 Mb）定位到2个主效QTL区间，区间内12个候选基因（Arahy.0S9GR8、Arahy.7D57NZ、Arahy.VXXK79、Arahy.D9MZDI、Arahy.CTM7LX、Arahy.8CP0UI、Arahy.J4K9CJ、Arahy.FEMB2S、Arahy.R1S0JT、Arahy.AV0RHQ、Arahy.EI43SS及Arahy.FVD5C0）主要涉及离子转运、细胞周期调控及细胞壁合成等生物学途径，可能参与花生种子大小形成。【结论】在A05染色体上定位到调控花生种子大小的稳定主效QTL区间，筛选获得12个候选基因，为花生高产分子育种提供了重要的基因资源和理论依据。
Abstract
[Purpose] This study aims to identify genetic loci closely associated with the physical trait of peanut seed size, providing theoretical basis and genetic resources for high-yield molecular breeding. [Methods] A total of 204 lines from a chromosomal segment substitution line (CSSL) population were employed in this study. The population was derived from a cross using "Qinhuangdaoliyang (QHDLY)" with large seeds as the male parent and "Pingnanshiyaodou (PNSYD)" with small seeds as the female parent. Mixed pools of large and small seeds were established, and physical traits related to peanut seed size were mapped through BSA-seq sequencing. Gene annotation was performed on candidate regions. [Results]A total of 713,242 SNPs and 218,258 InDel were detected in both parental lines and pooled pools. Four QTL regions associated with seed size traits were identified at the 95% confidence level (A05, A08, B05, B06). At the 99% confidence level, two major QTL interval was localized to chromosome A05 region 102.52~110.54 Mb (8.02 Mb) and B06 region 140.10 Mb~143.84 Mb（3.74 Mb）, encompassing 12 candidate genes (Arahy.0S9GR8, Arahy.7D57NZ, Arahy.VXXK79, Arahy.D9MZDI, Arahy.CTM7LX, Arahy.8CP0UI, Arahy.J4K9CJ, Arahy.FEMB2S, Arahy.R1S0JT, Arahy.AV0RHQ, Arahy.EI43SS, and Arahy.FVD5C0). These genes are primarily involved in biological pathways such as ion transport, cell cycle regulation, and cell wall synthesis, and may contribute to peanut seed size formation.[Conclusion]A major QTL interval for seed size stably existing on chromosome A05 was mapped and 12 candidate genes were obtained, which providing important theoretical basis and gene resources for molecular breeding of high-yield peanut.关键词
花生/种子大小/BSA-seq/染色体片段代换系/QTL定位Key words
Peanut/Seed Size/BSA-seq/CSSL/QTL Mapping引用本文复制引用
任常师,李泽仁,李松原,李楚珩,王嘉宝,杨鑫雷.基于BSA-seq的花生种子大小物理性状定位[EB/OL].(2026-04-21)[2026-04-22].http://www.paper.edu.cn/releasepaper/content/202604-154.学科分类
农作物/农业科学研究
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