大豆GmCCD8结瘤固氮功能验证
Functional Verification of Nodulation and Nitrogen Fixation of Soybean GmCCD8
付钊 1杜汇1
作者信息
- 1. 河北农业大学农学院，保定 071001
- 折叠
摘要
大豆是世界上重要的油料作物，在生产生活中多个领域均发挥着重要作用，我国作为大豆进口大国，每年大部分大豆都需要进口补充。氮素是植物所需的大量元素，在土壤中可利用氮素很少，因此大豆与根瘤菌共生固氮是大豆获取氮素的重要方式，通过根瘤菌将氮气还原为铵态氮、硝态氮供植株生长发育所利用，固氮过程不是植株或根瘤某一部分单独发挥作用，而是植株与根瘤多个组织器官、多种酶以及多个基因共同调控结瘤固氮进程，其中包括了结瘤信号识别、根瘤菌侵染、根瘤生长发育过程以及结瘤固氮等多个过程，这个过程是复杂且严密的。植株结瘤固氮能力提高，氮素获取量增加，对于提高植株产量具有重要影响。因此，研究大豆共生固氮的机制对于减少氮肥损耗、大豆产量提升、培育高固氮新品种等多个方面具有重要意义。本研究通过qRT-PCR分析基因时空表达模式，构建了过表达植株，通过改变基因在植株中表达量变化解释基因在结瘤固氮过程中的生物学功能。结果表明：类胡萝卜素裂解双加氧酶GmCCD8在根瘤中高效表达，GmCCD8在根瘤生长发育过程中发挥重要功能；通过除草剂、PCR技术获得稳定遗传的GmCCD8过表达株系（OX8a-1、OX8a-2和OX8a-3），通过分析植株表型发现，GmCCD8过表达植株株高、地上部鲜重、地上部干重、根鲜重和根干重方面均高于野生型植株，进一步研究发现，过表达植株酰脲也显著提高。
Abstract
Soybean is an important oil crop in the world and plays a significant role in various aspects of production and daily life. As a major importer of soybeans, China requires imports to meet most of its annual soybean demand. Nitrogen is a vital nutrient for plants, but the amount of available nitrogen in the soil is very limited. Therefore, symbiotic nitrogen fixation between soybean and rhizobia is an important way for soybeans to obtain nitrogen. Through rhizobia, atmospheric nitrogen is reduced to ammonium nitrogen and nitrate nitrogen for plant growth and development. The nitrogen fixation process is not performed by a single part of the plant or nodules alone but is regulated collectively by multiple plant and nodule tissues, various enzymes, and multiple genes. This includes processes such as nodule signal recognition, rhizobia infection, nodule growth and development, and nodule nitrogen fixation, making it a complex and precise process. Enhancing the plant\'s nodule nitrogen fixation capacity increases nitrogen acquisition, which significantly impacts yield improvement. Therefore, studying the mechanism of soybean symbiotic nitrogen fixation is of great significance for reducing nitrogen fertilizer loss, increasing soybean yield, and breeding new high nitrogen-fixation varieties. In this study, the spatiotemporal expression patterns of genes were analyzed using qRT-PCR, and overexpression plants were constructed. The biological function of genes in the nodulation nitrogen fixation process was elucidated by altering their expression levels in the plants. The results show that the carotenoid cleavage dioxygenase GmCCD8 is highly expressed in nodules and plays an important role in nodule growth and development. Stable genetically inherited GmCCD8 overexpression lines (OX8a-1, OX8a-2, and OX8a-3) were obtained using herbicides and PCR techniques. Phenotypic analysis demonstrated that GmCCD8 overexpression plants exhibited greater plant height, fresh and dry weight of aboveground parts, and fresh and dry weight of roots compared to wild-type plants. Further studies found that overexpression plants also showed a significant increase in urea levels.关键词
大豆/结瘤固氮/类胡萝卜素裂解双加氧酶Key words
Soybean/Nodulation nitrogen fixation/Carotenoid cleavage dioxygenase引用本文复制引用
付钊,杜汇.大豆GmCCD8结瘤固氮功能验证[EB/OL].(2026-04-27)[2026-04-28].http://www.paper.edu.cn/releasepaper/content/202604-198.学科分类
生物科学研究方法、生物科学研究技术/分子生物学/植物学
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