急性髓系白血病与淋巴瘤干性关键基因的研究
Research on Critical Stemness Genes in AML and Lymphoma
霍文慧 1游凤涛 2安钢力 1杨林3
作者信息
- 1. 苏州大学唐仲英血液学研究中心，苏州 215123
- 2. 博生吉医药科技（苏州）有限公司，江苏苏州 215123
- 3. 苏州大学唐仲英血液学研究中心，苏州 215123;博生吉医药科技（苏州）有限公司，江苏苏州 215123
- 折叠
摘要
目的：整合急性髓系白血病（Acute myeloid leukemia, AML）与弥漫大B细胞淋巴瘤（Diffuse large B-cell lymphoma, DLBCL）的转录组数据，构建多种机器学习预测模型并筛选关键基因，同时通过体外功能实验初步验证关键基因的作用。方法：从公共数据库获取AML及DLBCL多队列转录组数据，筛选差异基因，并构建101种机器学习算法组合的生存预测模型。针对模型筛选出的关键基因开展siRNA干扰实验，并通过RT-qPCR、Western Blot、流式细胞术及CAR-T细胞共培养杀伤实验进行功能验证。结果：PLA2G4A和LGALS2在多种算法中均被筛选为核心特征基因；体外功能实验表明，敲低PLA2G4A或LGALS2可在部分细胞系中降低Bcl-2表达、上调Caspase3水平，并增强肿瘤细胞对免疫细胞杀伤的敏感性。结论： PLA2G4A和LGALS2具有作为潜在诊断标志物和治疗靶点的价值，为理解AML与DLBCL的病理机制提供了新的研究视角。
Abstract
Objective: Integrate transcriptomic data of AML and DLBCL to construct machine learning models for key gene screening and preliminary in vitro validation. Methods: Multi-cohort transcriptomic data were obtained from public databases. Differential gene analysis and 101 machine learning combinations were performed for survival prediction. Key genes were validated by siRNA knockdown, RT-qPCR, Western Blot, flow cytometry, and CAR-T killing assays. Results: PLA2G4A and LGALS2 were identified as core signature genes. Their knockdown reduced Bcl-2, upregulated Caspase3, and enhanced tumor sensitivity to immune killing. Conclusions: PLA2G4A and LGALS2 represent potential diagnostic biomarkers and therapeutic targets, providing novel insights into AML and DLBCL pathogenesis.关键词
肿瘤学/急性髓系白血病/淋巴瘤/肿瘤干性Key words
Oncology/Acute Myeloid Leukemia/Lymphoma/Cancer Stemness引用本文复制引用
霍文慧,游凤涛,安钢力,杨林.急性髓系白血病与淋巴瘤干性关键基因的研究[EB/OL].(2026-04-28)[2026-04-29].http://www.paper.edu.cn/releasepaper/content/202604-206.学科分类
遗传学/细胞生物学/生物科学研究方法、生物科学研究技术
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