BACH1表达在砷致HaCaT细胞恶性增殖中的动态变化
Dynamic Changes in BACH1 Expression during Arsenic-Induced Malignant Proliferation of HaCaT Cells
摘要
【目的】本研究旨在探讨BACH1在砷诱导HaCaT细胞恶性增殖过程中的动态变化。【方法】收集0.1 μM NaAsO2长期处理致HaCaT细胞恶性增殖过程各关键代数（0、1、7、14、21、28、35代）的细胞，Rt-qPCR和western blot实验用于检测各关键检测带你BACH1的动态表达，线性回归模型用于趋势分析。【结果】随着染砷代数增加，BACH1的mRNA及蛋白水平呈显著的线性升高趋势（P<0.05）。【结论】在砷诱导HaCaT细胞恶性增殖过程中BACH1的表达呈持续升高趋势，可能促进了HaCaT细胞的恶性增殖。
Abstract
【Objective】This study aims to investigate the dynamic changes of BACH1 during arsenic-induced malignant proliferation of HaCaT cells.【Methods】HaCaT cells at key passages (0, 1, 7, 14, 21, 28, and 35) were collected during the process of malignant proliferation induced by long-term treatment with 0.1 μM NaAsO?. RT-qPCR and western blot were used to detect the dynamic expression of BACH1 at each key time point, and a linear regression model was applied for trend analysis.【Results】With the increase of exposure passages, both the mRNA and protein levels of BACH1 showed a significant linear upward trend (P < 0.05).【Conclusion】During arsenic-induced malignant proliferation of HaCaT cells, the expression of BACH1 continuously increases, which may promote the malignant proliferation of HaCaT cells.关键词
BACH1、砷、HaCaT、动态表达Key words
BACH1、Arsenic/、HaCaT/、Dynamic expression引用本文复制引用
贾浩奇,安艳.BACH1表达在砷致HaCaT细胞恶性增殖中的动态变化[EB/OL].(2026-04-29)[2026-04-30].http://www.paper.edu.cn/releasepaper/content/202604-209.学科分类
细胞生物学/生物科学研究方法、生物科学研究技术
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