基于CD33纳米抗体的CAR-γδT细胞对急性髓系白血病的抗肿瘤作用研究
Antitumor Activity of CD33 Nanobody-Based CAR-γδT Cells in Acute Myeloid Leukemia
黄永康 1游凤涛 2姜丽翠 2安钢力 1杨林3
作者信息
- 1. 苏州大学唐仲英血液学研究中心，苏州 215123
- 2. 博生吉医药科技（苏州）有限公司，江苏苏州 215123
- 3. 苏州大学唐仲英血液学研究中心，苏州 215123;博生吉医药科技（苏州）有限公司，江苏苏州 215123
- 折叠
摘要
目的：本研究旨在通过优化γδT细胞制备和扩增流程，构建基于CD33纳米抗体的CAR-γδT细胞，通过体外实验评估其抗肿瘤活性。方法：通过慢病毒转染构建四种CD33?CAR?γδT细胞，使用流式细胞术检测转染效率、体外抗肿瘤活性、细胞因子释放、分化表型以及扩增能力进行检测。结果：制备高滴度的慢病毒，成功构建CD33-CAR-γδT细胞，在体外验证其具有良好的抗肿瘤活性。结论：本研究成功构建四种结构的CD33-CAR-γδT细胞，四种细胞均具备体外抗肿瘤活性，其中BC结构表现最优，在记忆表型维持（高TNaive比例）及持续杀伤能力上显著优于其他结构。
Abstract
Objective: This study aimed to construct CD33 nanobody-based CAR?γδT cells by optimizing the preparation and expansion protocols of γδT cells, and to evaluate their anti?tumor activity in vitro. Methods: Four types of CD33-CAR-γδT cells were constructed via lentiviral transfection, and flow cytometry was performed to detect transfection efficiency, in vitro anti?tumor activity, cytokine release, differentiation phenotype, and expansion capacity. Results: High-titer lentiviruses were prepared, and CD33-CAR-γδT cells were successfully constructed and verified to exhibit favorable anti-tumor activity in vitro. Conclusion: Four structurally distinct CD33-CAR-γδT cells were successfully established in this study, all of which exerted in vitro anti?tumor activity; among them, construct BC showed the optimal performance, with memory phenotype maintenance (high proportion of naive T cells), and sustained cytotoxicity compared with the other structures.关键词
急性髓系白血病/CD33/γδT细胞/CAR-T细胞Key words
AML/CD33/γδT cell/CAR-T cell引用本文复制引用
黄永康,游凤涛,姜丽翠,安钢力,杨林.基于CD33纳米抗体的CAR-γδT细胞对急性髓系白血病的抗肿瘤作用研究[EB/OL].(2026-05-08)[2026-05-10].http://www.paper.edu.cn/releasepaper/content/202605-5.学科分类
肿瘤学/医学研究方法/基础医学
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