低磷胁迫下不同基因型陆地棉根组织转录组学分析
Transcriptomic profiling of root systems in different Gossypium hirsutum genotypes under low phosphorus stress
袁圣雪 1孙正文1
作者信息
- 1. 河北农业大学农学院/华北作物改良与调控国家重点实验室/河北省作物种质资源重点实验室，保定 071001
- 折叠
摘要
【目的】为了探讨苗期不同基因型陆地棉对低磷胁迫的响应。【方法】以棉花耐低磷品种PT80和低磷敏感品种PS187为试验材料，对棉花幼苗根系进行不同磷处理的转录组测序分析。【结果】测序结果表明：在低磷胁迫条件下，两个品种间响应低磷胁迫差异表达的基因共1659个，且这些差异基因大部分表现为上调表达。GO富集结果显示，差异表达基因主要富集到液泡膜、各种氧化还原酶活性等功能。KEGG的富集结果显示，差异表达基因主要富集到代谢、次生代谢物的生物合成、苯丙烷类生物合成、植物激素信号转导等信号通路中。【结论】本研究系统解析陆地棉苗期响应低磷胁迫的遗传调控网络，深入阐释其磷素高效利用的分子基础。对比低磷响应差异基因的相对表达量和表达模式，挖掘出6个调控陆地棉耐低磷性的关键候选基因。RT-qPCR分析表明，6个候选基因与转录组测序结果基本一致。
Abstract
[Objective]To investigate the response of different phosphorus (P) efficiency genotypes of upland cotton to low phosphorus stress during the seedling stage. [Method] We used the cotton variety PT80, which is tolerant to low phosphorus, and the variety PS187, which is sensitive to low phosphorus, as experimental materials. [Result] Transcriptome sequencing analysis was conducted on the root systems of cotton seedlings subjected to low phosphorus treatment. The sequencing results indicated that under low phosphorus (LP) stress conditions, there were 1659 differentially expressed genes (DEGs) between the two varieties, most of which were upregulated. GO enrichment analysis revealed that the differentially expressed genes were primarily enriched in functions such as vacuole membrane and various oxidoreductase activities. KEGG enrichment analysis showed that the differentially expressed genes were mainly enriched in pathways related to metabolites, the biosynthesis of secondary metabolites, biosynthesis of phenylpropanoids, and plant hormone signal transduction. [Conclusion] This study systematically elucidated the genetic regulatory network of upland cotton seedlings in response to low phosphorus stress and provides an in-depth explanation of the molecular basis for efficient phosphorus utilization. By comparing the relative expression levels and expression patterns of differential genes in the low phosphorus stress, six key candidate genes regulating the low phosphorus tolerance of upland cotton were identified. RT-qPCR analysis confirmed that the expression patterns of the randomly selected six genes were consistent with the transcriptome sequencing results.关键词
陆地棉/低磷胁迫/转录组分析Key words
Cotton/Low phosphorus stress/Transcriptome analysis引用本文复制引用
袁圣雪,孙正文.低磷胁迫下不同基因型陆地棉根组织转录组学分析[EB/OL].(2026-05-08)[2026-05-10].http://www.paper.edu.cn/releasepaper/content/202605-6.学科分类
分子生物学
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