MYPT1敲降增强炎症反应的研究
A Study on the Role of MYPT1 Knockdown in Enhancing Inflammatory Responses
肖锐 1何伟奇1
作者信息
- 1. 苏州大学苏州医学院剑桥-苏大基因组资源中心，江苏省苏州市 215123
- 折叠
摘要
MYPT1作为肌球蛋白磷酸酶的调节亚基，参与调控多种生理过程，然而其与炎症反应之间的关联及作用机制目前尚缺乏研究。为探究MYPT1在炎症调控中的潜在功能，本研究构建了MYPT1稳定敲降的L929细胞系（shMYPT1）。通过转录组测序及GSEA富集分析筛选差异表达基因，并结合Western Blot、免疫荧光及qRT-PCR在蛋白和mRNA水平进行验证。GSEA分析显示，shMYPT1细胞中NF-κB及MAPK信号通路相关基因集显著富集，且两通路在蛋白水平上被显著激活。同时，下游炎症通路基因集同样显著富集，qRT-PCR验证显示，多个关键炎症基因mRNA表达水平显著升高。本研究表明，L929细胞中MYPT1敲降通过协同激活NF-κB和MAPK信号通路，上调下游炎症因子表达水平。这揭示了MYPT1功能缺失促发炎症反应，为炎症相关疾病的治疗提供了潜在新靶点与思路。
Abstract
MYPT1, as a regulatory subunit of myosin phosphatase, is involved in the regulation of various physiological processes; however, its association with inflammatory responses and the underlying mechanisms remain poorly understood. To investigate the potential role of MYPT1 in inflammation regulation, this study established an L929 cell line with stable MYPT1 knockdown (shMYPT1). Differentially expressed genes were identified through transcriptomic sequencing and GSEA enrichment analysis, and validated at the protein and mRNA levels using Western blot, immunofluorescence, and qRT-PCR. GSEA analysis revealed significant enrichment of gene sets associated with the NF-κB and MAPK signaling pathways in shMYPT1 cells, and both pathways were significantly activated at the protein level. Concurrently, gene sets associated with downstream inflammatory pathways were also significantly enriched. qRT-PCR validation revealed that mRNA expression levels of multiple key inflammatory genes were significantly elevated. This study demonstrates that MYPT1 knockdown in L929 cells upregulates the expression of downstream inflammatory factors by synergistically activating the NF-κB and MAPK signaling pathways. This finding reveals that MYPT1 functional loss triggers inflammatory responses, providing potential new targets and therapeutic strategies for the treatment of inflammation-related diseases.关键词
MYPT1/NF-κB信号通路/MAPK信号通路/炎症反应Key words
MYPT1/NF-κB signaling pathway/MAPK signaling pathway/inflammatory response引用本文复制引用
肖锐,何伟奇.MYPT1敲降增强炎症反应的研究[EB/OL].(2026-05-11)[2026-05-13].http://www.paper.edu.cn/releasepaper/content/202605-27.学科分类
基础医学
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