烟草MYC家族生物信息学及表达水平分析
The bioinformatics and expression analysis of the MYC family in Nicotiana tabacum
张珏茹 1王冰儒 1邓可宣1
作者信息
- 1. 西南大学农学与生物科技学院，重庆 400715
- 折叠
摘要
MYC转录因子在植物激素信号传导、逆境胁迫应答及次生代谢调控中发挥关键作用。为明确烟草MYC基因家族的结构特征与激素响应模式，本研究采用生物信息学方法对烟草MYC家族进行鉴定，并系统分析其理化性质、保守基序、基因结构、系统进化关系及启动子顺式作用元件；实时荧光定量PCR结果表明，在病原菌侵染胁迫下，8个受试候选NtMYCs基因呈现出显著的表达模式分化。其中，NtMYC43与NtMYC28的相对表达量极显著上调至对照组的215倍和61倍，表现出强烈的正向转录应答；NtMYC3、NtMYC11等4个成员也呈显著上调趋势；而NtMYC25与NtMYC4表达无显著差异。该表达特征与其启动子区富集的防御应激元件相吻合。综上所述，烟草MYC家族在响应生物胁迫时存在明显的功能分化，受强烈诱导的核心成员可能作为关键的转录激活因子参与烟草早期抗病防御网络。本研究为深入解析烟草抗病机制及分子抗逆育种提供了重要的理论依据与候选基因。
Abstract
MYC transcription factors play key roles in plant hormone signal transduction, stress response, and secondary metabolism regulation. To clarify the structural characteristics and hormone response patterns of the MYC family in tobacco, a bioinformatic approach was used to identify the MYC family members in tobacco and systematically analyze their physicochemical properties, conserved motifs, gene structure, phylogenetic relationships, and promoter cis-acting elements.Quantitative real-time PCR (qRT-PCR) analysis revealed a significant divergence in the expression patterns of the eight candidate NtMYC genes in response to pathogen infection. Notably, NtMYC43 and NtMYC28 exhibited a robust positive transcriptional response, with their relative expression levels dramatically upregulated by 215-fold and 61-fold compared to the control, respectively. Four other members, including NtMYC3 and NtMYC11, also displayed significant upregulation trends, whereas the expression of NtMYC25 and NtMYC4 remained largely unaltered. These expression profiles are highly consistent with the enrichment of defense- and stress-responsive cis-elements in their promoter regions. Taken together, these findings demonstrate a distinct functional divergence within the tobacco MYC family under biotic stress. The strongly induced core members likely function as crucial transcriptional activators orchestrating early disease defense networks. Ultimately, this study provides valuable candidate genes and fundamental theoretical insights for elucidating tobacco disease resistance mechanisms and advancing molecular breeding for stress tolerance.关键词
烟草/MYC转录因子/生物信息学/实时荧光定量PCRKey words
Nicotiana tabacum/Calmodulin-binding transcriptional activator (MYC)/Bioinformatics/Real-time fluorescence quantitative PCR引用本文复制引用
张珏茹,王冰儒,邓可宣.烟草MYC家族生物信息学及表达水平分析[EB/OL].(2026-05-19)[2026-05-20].http://www.paper.edu.cn/releasepaper/content/202605-86.学科分类
农业科学研究/生物科学研究方法、生物科学研究技术/遗传学/分子生物学
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