具有优异类过氧化物酶活性的Co1Fe1-MOF用于H2O2和葡萄糖的比色检测
Co1Fe1-MOF with exceptional peroxidase-like activity for colorimetric detection of H2O2 and glucose
摘要
金属有机框架（MOFs）凭借比表面积高及活性位点丰富等特点，已成为纳米酶领域的研究热点。然而，针对单金属Co-MOF类过氧化物酶活性有限的问题，在Co-MOF中引入过渡金属Fe合成二维双金属Co1Fe1-MOF。利用Co与Fe之间的电子协同效应显著增强材料的类过氧化物酶活性。同时其二维纳米片结构使材料表面的活性位点充分暴露，进一步提升催化反应速率。基于Co1Fe1-MOF优异的类酶催化活性，构建了级联催化比色传感平台，应用于H2O2和葡萄糖的灵敏比色检测。该比色传感平台对H2O2和葡萄糖具有较宽的线性检测范围，检测限分别低至0.077 μM和0.3 μM。并且具有良好的稳定性和抗干扰能力，可用于实际样品中葡萄糖的检测。
Abstract
Metal-organic frameworks (MOFs) have become a research hotspot in the field of nanoenzymes due to their high specific surface area and rich active sites. However, in order to solve the problem of limited activity of monometallic Co-MOF peroxidase enzymes, the transition metal Fe was introduced into Co-MOF to synthesize two-dimensional bimetallic Co1Fe1-MOF. The electron synergy between Co and Fe was used to significantly enhance the peroxidase-like activity of the material. At the same time, its two-dimensional nanosheet structure fully exposes the active sites on the surface of the material, further increasing the catalytic reaction rate. Based on the excellent enzyme-like catalytic activity of Co1Fe1-MOF, a cascade catalytic colorimetric sensing platform was constructed for the sensitive colorimetric detection of H2O2 and glucose. The colorimetric sensing platform has a wide linear detection range for H2O2 and glucose, with detection limits as low as 0.077 M and 0.3 M. It exhibits excellent stability and robust anti-interference performance, which can be used for the detection of glucose in actual samples.关键词
双金属MOF纳米酶/比色检测/H2O2/葡萄糖Key words
Bimetallic MOF nanoenzyme/Colorimetric detection/H2O2/Glucose引用本文复制引用
赵彤,陈艳伟.具有优异类过氧化物酶活性的Co1Fe1-MOF用于H2O2和葡萄糖的比色检测[EB/OL].(2026-05-22)[2026-06-10].https://chinaxiv.org/abs/202605.00178.学科分类
生物科学研究方法、生物科学研究技术/生物化学
