小麦籽粒发育过程中代谢物动态变化研究
Analysis of dynamic metabolite changes during the developmental process of wheat (Triticum aestivum L.) kernel
陈良 1张硕 1刘冬成1
作者信息
- 1. 河北农业大学农学院/省部共建华北作物改良与调控国家重点实验室，河北保定，071000
- 折叠
摘要
小麦籽粒的发育过程直接决定了其最终的产量和品质。因此，系统性解析代谢动态变化网络是深入研究品质形成机理的关键。本研究采用非靶向代谢组学的方法，分析石家庄8号背景下，花后10、15、20、25、30和40天籽粒代谢物的动态特征，旨在系统阐明籽粒发育过程中代谢物的时序性动态变化。以花后10天籽粒为对照组，花后15、20、25、30和40天的差异代谢物（DAMs）数目分别为311、330、340、398和384个，呈逐步递增后趋于稳定的动态变化模式。时序性分析显示，52种保守代谢物贯穿籽粒全发育时期，并且各时期鉴定到32~51个特异性代谢物。KEGG通路富集分析显示，苯丙烷类生物合成通路在花后15天和20天显著富集，花后25天和30天逐步转向糖酵解/糖异生、淀粉与蔗糖代谢等通路，花后40天则富集于泛醌及其它萜类醌和核苷酸代谢等相关通路。这种代谢网络的时序性转换揭示了籽粒发育过程中防御与结构建成、物质合成积累及品质形成的生物学过程。
Abstract
The development process of wheat grains directly determines their final yield and quality. Therefore, systematic dissection of the dynamic metabolic network is crucial for in-depth investigation of the mechanisms underlying quality formation. In this study, an untargeted metabolomics approach was employed to analyze the dynamic characteristics of metabolites in grains of wheat cultivar Shijiazhuang 8 at 10, 15, 20, 25, 30, and 40 days after anthesis, aiming to systematically elucidate the temporal dynamic changes of metabolites during grain development. Using grains at 10 days after anthesis as the control group, the numbers of differentially accumulated metabolites (DAMs) at 15, 20, 25, 30, and 40 days after anthesis were 311, 330, 340, 398, and 384, respectively, showing a dynamic pattern of gradual increase followed by stabilization. Temporal analysis revealed that 52 conserved metabolites were present throughout the entire grain development period, while 32 to 51 specific metabolites were identified at each stage. KEGG pathway enrichment analysis showed that the phenylpropanoid biosynthesis pathway was significantly enriched at 15 and 20 days after anthesis. At 25 and 30 days after anthesis, the enriched pathways gradually shifted to glycolysis/gluconeogenesis, starch and sucrose metabolism, and related pathways. At 40 days after anthesis, enriched pathways included ubiquinone and other terpenoid-quinone biosynthesis, nucleotide metabolism, and related pathways. Such temporal transitions in the metabolic network reflect the biological processes of defense and structural construction, biosynthesis and accumulation of substances, and quality formation during wheat grain development.关键词
小麦/代谢组学/动态变化/差异代谢物/Key words
wheat/metabolomics/dynamic changes/differentially accumulated metabolites/引用本文复制引用
陈良,张硕,刘冬成.小麦籽粒发育过程中代谢物动态变化研究[EB/OL].(2026-05-25)[2026-05-26].http://www.paper.edu.cn/releasepaper/content/202605-107.学科分类
农业科学研究/生物化学
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