蛋白激酶Fer在电离辐射诱导小鼠生精功能损伤中的作用研究
Role of Protein Kinase Fer in Ionizing Radiation-Induced SpermatogenicDysfunction in Mice
高稳 1狄青 1穆荣素 1吴潇 1陈娜1
作者信息
- 1. 苏州大学放射医学与防护学院，苏州，215123
- 折叠
摘要
目的：探究酪氨酸激酶Fer在电离辐射诱导雄性生殖损伤中的作用。方法：采用X射线局部分次照射C57BL/6J小鼠下腹部（0.5 Gy/d×4 d，总剂量2 Gy），评估不同时间点的生殖损伤；利用蛋白质组学筛选差异蛋白，并进行Western blot验证；在小鼠精原干细胞减数分裂时期抑制Fer活性，观察其对Ing2表达水平的影响。结果：照射后第1天小鼠出现急性生殖损伤，第35天发展为暂时性不育，第70天恢复。睾丸组织中的Fer和Ing2在三个时间点的表达呈"下降-升高-恢复"动态变化。精原干细胞中抑制Fer活性后Ing2表达下调。结论：Fer可能通过调节减数分裂过程，在电离辐射诱导的小鼠生殖损伤及恢复过程发挥重要作用。
Abstract
Objective: To investigate the role of tyrosine kinase Fer in ionizing radiation-induced male reproductive injury. Methods: C57BL/6J mice were exposed to fractionated local X-ray irradiation to the lower abdominal region (0.5 Gy/d for 4 d, total dose 2 Gy). Reproductive injury was evaluated at different time points. Differential proteins were screened using proteomics and validated by Western blot. Fer activity was inhibited during meiosis in mouse spermatogonial stem cells to observe its effect on Ing2 expression. Results: Mice exhibited acute reproductive injury on day 1 post-irradiation, developed temporary infertility on day 35, and recovered by day 70. The expression levels of Fer and Ing2 in testicular tissue showed a dynamic "decrease-increase-recovery" pattern across the three time points. Inhibition of Fer activity in spermatogonial stem cells led to downregulation of Ing2 expression. Conclusion: Fer may play an important role in ionizing radiation-induced reproductive injury and recovery in mice by regulating the meiotic process.关键词
放射医学/蛋白激酶Fer/减数分裂/生殖损伤Key words
Radiological medicine/Protein kinase Fer/Meiosis/Reproductive injury引用本文复制引用
高稳,狄青,穆荣素,吴潇,陈娜.蛋白激酶Fer在电离辐射诱导小鼠生精功能损伤中的作用研究[EB/OL].(2026-05-27)[2026-05-28].http://www.paper.edu.cn/releasepaper/content/202605-124.学科分类
细胞生物学/分子生物学
