GhnsLTPsA10互作蛋白的筛选及其在棉花抗黄萎病中的功能解析
Genome-wide Identification of Screening of GhnsLTPsA10 Interaction Proteins and Their Functional Analysis in Cotton Resistance to Verticillium Wilt
刘雨萱 1张艳 1王志诚 1解美霞 1焦梦佳 1王岩 1谷曼 1王欣雨 1王珊珊 1马峙英1
作者信息
- 1. 华北作物改良与调控国家重点实验室/河北农业大学农学院
- 折叠
摘要
棉花作为全球重要的经济作物之一，在生产中占据重要地位，而黄萎病是棉花大田生产中最严重的病害。植物非特异性脂质转运蛋白（nsLTPs）能够在病原菌的诱导下大量表达，使植物发挥防御作用。本研究利用酵母双杂交技术筛选出GhnsLTPsA10互作蛋白GhPIP2，并通过超表达拟南芥、VIGS试验证明GhPIP2正向调控棉花抗黄萎病，活性氧相关物质的测定结果表明GhnsLTPsA10通过介导活性氧抗病途径来正向调控棉花对黄萎病的抗性。本研究明确了GhnsLTPsA10互作蛋白的网络调控关系，揭示其调控棉花抗黄萎病中的内在分子机制，为后续深入阐明其分子功能及作物遗传改良提供理论依据。
Abstract
As one of the world\'s important economic crops, cotton holds a significant position in production, while Verticillium wilt is the most severe disease in cotton field production. Plant nonspecific lipid transfer proteins (nsLTPs) can be highly expressed under the induction of pathogens, enabling plants to exhibit defensive functions. In this study, the GhnsLTPsA10 interacting protein GhPIP2 was screened using yeast two-hybrid technology, and it was demonstrated through overexpression in Arabidopsis and VIGS experiments that GhPIP2 positively regulates cotton resistance to Verticillium wilt. Measurement of reactive oxygen species-related substances indicated that GhnsLTPsA10 positively regulates cotton\'s resistance to Verticillium wilt by mediating the reactive oxygen-mediated defense pathway. This study clarifies the network regulatory relationship of GhnsLTPsA10 interacting proteins and reveals the intrinsic molecular mechanism of its regulation of cotton resistance to Verticillium wilt, providing a theoretical basis for subsequent in-depth elucidation of its molecular function and crop genetic improvement.关键词
棉花/黄萎病/nsLTPs/PIPs/活性氧Key words
Cotton/Verticillium wilt/nsLTPs/PIPs/ROS引用本文复制引用
刘雨萱,张艳,王志诚,解美霞,焦梦佳,王岩,谷曼,王欣雨,王珊珊,马峙英.GhnsLTPsA10互作蛋白的筛选及其在棉花抗黄萎病中的功能解析[EB/OL].(2026-05-28)[2026-06-03].http://www.paper.edu.cn/releasepaper/content/202605-131.学科分类
植物保护/农作物
