改良圆环滤纸法在长角血蜱驱避反应测试中的应用
Application of the modified circular filter paper method for repellency bioassays of Haemaphysalis longicornis
李柏雅 1白晓雅 1郭紫莲 1辛正 2陈泽1
作者信息
- 1. 河北师范大学
- 2. 济南市疾病预防控制中心
- 折叠
摘要
摘要：目的 鉴于目前已有的测试蜱驱避率的方法在稳定性、观测时长及药效比较方面不适合长角血蜱的测评，本研究通过优化圆环滤纸法的施药宽度、空白区宽度、试虫种群的龄期、滤纸干燥时间等参数，以市面上常用驱避剂羟哌酯作为标准品，建立一套适合长角血蜱的驱避测试方法。方法 以实验室长期驯化的 10-25 日龄的长角血蜱若蜱为测试对象，分别采用目前常用的圆环滤纸法和我们优化参数后的改良改良圆环滤纸法评估20%羟哌酯的驱避效果；进一步通过改良圆环滤纸法评估20% 羟哌酯的有效作用时间及不同浓度（5%、8%、10%、15%和20%）羟哌酯对长角血蜱若蜱的驱避效果。结果 在相同施药面积和试虫批次下，20%羟哌酯的驱避率因测试方法差异显著：常用的圆环滤纸法在1、3、5 min的平均驱避率分别为87%、68%、47%，且仅1 min时变异系数较小（7.76%），3 min和5 min分别升至16.7%和48.15%，稳定性差；改良圆环滤纸法对应驱避率为99%、99%、93%，变异系数分别为3.19%、3.19%和7.26%，均远低于10%，结果稳定。通过改良圆环滤纸法测试药效时长发现，20%羟哌酯在第6小时仍对长角血蜱若蜱具有良好驱避活性，前6小时每小时驱避率分别为93.3%、86.7%、83.3%、80%、76.7%、70%。不同浓度羟哌酯（5%、8%、10%、15%、20%）测试显示，随浓度增加，其对长角血蜱若蜱的驱避率逐渐上升，分别为26.7%、56.7%、73.3%、86.7%、93.3%。结论 本研究建立的改良圆环滤纸法不仅具有操作简单、稳定性强、重复性高、灵敏度适中的特点，同时又能充分体现不同浓度驱避剂的驱避梯度，可用于驱避剂对长角血蜱若蜱驱避效果的测定与筛选。
Abstract
Abstract:Objective In view of the fact that the existing methods for testing tick repellency rate are not suitable for the evaluation of Haemaphysalis longicornis in terms of stability, observation duration and efficacy comparison, this study optimized parameters including the application width of the circular filter paper method, the width of the blank zone, the developmental stage of test ticks, and the filter paper drying time. Using the commonly marketed repellent icaridin as the standard substance, a repellency test method suitable for Haemaphysalis longicornis was established.Methods Nymphs of Haemaphysalis longicornis acclimated in the laboratory for 1025 days were selected as test specimens. The repellent efficacy of 20% picaridin was assessed with the conventional circular filter paper method and our modified version of this assay with optimized parameters. We further used the modified circular filter paper method to test the effective protection duration of 20% picaridin, and compared repellent performance of picaridin at serial concentrations (5%, 8%, 10%, 15%, and 20%) against H. longicornis nymphs.Results Under the same application area and test-tick batch, the repellency of 20% icaridin varied markedly with test method. The conventional ring filter paper method gave mean repellency rates of 87%, 68% and 47% at 1, 3 and 5 min. The coefficient of variation was low only at 1 min (7.76%) and rose to 16.7% and 48.15% at 3 and 5 min, so stability was poor. The modified ring filter paper method gave 99%, 99% and 93%, with coefficients of variation of 3.19%, 3.19% and 7.26%, all well below 10%, and the results were stable. Efficacy duration tested by the modified method showed that 20% icaridin still repelled Haemaphysalis longicornis nymphs well at 6 h, with hourly repellency rates of 93.3%, 86.7%, 83.3%, 80%, 76.7% and 70% over the first 6 h. At 5%, 8%, 10%, 15% and 20% icaridin, repellency against H. longicornis nymphs rose with concentration, to 26.7%, 56.7%, 73.3%, 86.7% and 93.3%, respectively.Conclusion The modified circular filter paper method established in this study features simple operation, strong stability, high repeatability and moderate sensitivity. Meanwhile, it can well reflect the repellency gradient of repellents at different concentrations, and can be used for the determination and screening of the repellent effect of repellents against nymphs of Haemaphysalis longicornis.关键词
关键词：长角血蜱/若蜱/羟哌酯/圆环滤纸法/改良圆环滤纸法Key words
Keywords Haemaphysalis longicornis/nymphs/icaridin/circular filter paper method/modified circular filter paper method引用本文复制引用
李柏雅,白晓雅,郭紫莲,辛正,陈泽.改良圆环滤纸法在长角血蜱驱避反应测试中的应用[EB/OL].(2026-10-30)[2026-11-02].https://chinaxiv.org/abs/202610.00240.学科分类
医学研究方法
