小麦生育后期叶片和籽粒应答供氮水平的转录谱特征研究

Using leaves and grains during late growth stage of wheat (cv. Jimai 22) as materials, the differentially expressed genes (DEGs) in above organs in response to N starvation stress were identified based on high-throughput RNAseq sequencing technique together with corresponding bioinformatic tools. Results indicated that compared with control (N240), totally 1835genes were shown to be differentially expressed in leaves under low-N stress treatment, including that 533 were up-regulated and 1302 were down-regulated. GO functional annotation and KEGG analyses indicated that the above differentially expressed genes were mainly involved in biological processes, cell components, and molecular functions and mainly enriched in signaling or biochemical pathways associated with mTOR signaling pathway, PI3K-Akt signaling pathway, Th1 and Th2 cell differentiation, carbon fixation in photosynthetic organisms, and starch and sucrose metabolism. Likewise, a total of 410 genes were found to be differentially expressed in grains that were treated by N starvation stress, of which, 223 were up-regulated and 187 were down-regulated. These differentially expressed genes are mainly divided into three categories including biological process, cell component and molecular function and overrepresented by those involved in MTOR signaling pathway, PI3K-Akt signaling pathway, Th1 and Th2 cell differentiation and bosynthesis of phenylpropane. Our results suggested that the responses of leaves and grains to external N starvation stress are closely associated with the modulation on transcription efficiency of a quantity of genes related to cellular structure, molecular function, and biological processes.