基于DNA为模板的铜银纳米簇检测汞离子方法研究
etection of Mercury ions Based on DNA-templated Copper/Silver Nanoclusters
本文制备了以DNA为模板的铜/银纳米簇（DNA-CuAgNCs）。Hg2+可与制备的DNA-CuAgNCs特异性结合而使其荧光猝灭，基于此建立了荧光传感方法。DNA-Cu/AgNCs荧光猝灭程度与Hg2+有良好的线性关系，在1.0 nM~200nM范围内的线性方程为Y = 3.8814 [Hg2+] + 11.0544（R2 = 0.9933），检出限为0.09nM。湖水样品加标回收率在107.6%~117.3%之间，相对标准偏差在3.1%~8.5%之间。该方法具有操作简单、成本较低、灵敏度高等优点。
In this study, copper/silver nanoclusters (DNA-CuAgNCs) using DNA as a template were synthesized.Hg2+ can specifically bind to DNA-CuAgNCs to quench its fluorescence, and based on this, a fluorescence sensing method is established.The fluorescence quenching degree of DNA-CuAgNCs has a good linear relationship with Hg2+. The linear equation in the concentrationrange of 1.0 nM to 200nM is Y = 3.8814 [Hg2+] + 11.0544 (R2 = 0.9933), and the limit of detection is 0.09nM.The recoveries of lake water samples was from 107.6% to 117.3%, and the relative standard deviation(RSD) was from 3.1% to 8.5%. The method has the advantages of simple operation, low cost and high sensitivity.
钱志娟、任冰雪、彭池方
环境污染、环境污染防治化学环境生物学
荧光检测金属纳米簇汞离子
fluorescent nanomaterialsmetal nanoparticlesmercury ions
钱志娟,任冰雪,彭池方.基于DNA为模板的铜银纳米簇检测汞离子方法研究[EB/OL].(2019-01-25)[2025-08-02].http://www.paper.edu.cn/releasepaper/content/201901-180.点此复制
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