铀和铅对枯草杆菌毒性的研究
oxicity of Uranium and Lead to B.subtilis
枯草杆菌在处理重金属废水领域用着广泛的应用前景,研究重金属对枯草杆菌的最大无作用剂量和致死浓度是非常必要的。本实验采用了3种方法研究了铀和铅这两种重金属对枯草杆菌的毒性:1)试纸法测抑菌圈的直径;2)血球计数板测细菌菌数;3)高倍显微镜下观察细菌形态和密度的变化。实验结果表明:铀和铅对枯草杆菌的生长繁殖有着显著的影响。铀和铅对枯草杆菌的最大无作用剂量均小于1mg/L,铀对枯草杆菌的致死浓度为500mg/L,铅对枯草杆菌的致死浓度为1000mg/L。同时,还对以上这3种方法进行了比较,确定了其各自的应用范围,为重金属对微生物毒性研究方法的选择提供了参考。
B.subtilis has the broad foreground in dealing with the heavy metals waste water. It is necessary to study the toxicity of heavy metals to B.subtilis and get “the Maximum No-Effect Level ” and “the Lethal Concentration”.The toxicity of different concentration Uranium and Lead to B.subtilis in its growth was studied by three different methods in terms of measuring the diameter of the circle of bacteriostasis, counting the bacterial population by Heamacytometer and observing the change of the shape and the density of the bacteria through microscope. The experimental results have indicated that these two heavy metals had great inhibition effect to the growth of B.subtilis. The Maximum No-Effect Level of Uranium and Lead to B.subtilis are below 1mg/L and the Lethal Concentration of Uranium and Lead to B.subtilis are 500mg/L and 1000mg/L respectively. In these methods used for the toxicity of heavy metals to microorganism,counting the bacterial population are more sensitive.
招国栋、徐伟昌、赵由才、刘清
环境污染、环境污染防治生物科学研究方法、生物科学研究技术环境生物学
毒性 枯草杆菌 铀 铅
toxicityB.subtilisUraniumLead
招国栋,徐伟昌,赵由才,刘清.铀和铅对枯草杆菌毒性的研究[EB/OL].(2007-04-05)[2025-08-02].http://www.paper.edu.cn/releasepaper/content/200704-146.点此复制
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