XCR4在小脑颗粒神经元发育过程中的作用

Objective: To investigate the effect of chemokine receptor CXCR4 on the proliferation and migration of granule neuron precursor cells(GNPs) during cerebellar development. Methods: In this study, Cell biology, molecular biology, and conditional knockout mice(Math1-Cre/CXCR4 flox/flox) were used to detect the expression and effects of CXCR4 on cerebellar development. Results: 1) The expression of CXCR4 is highest on P4, then gradually decreases with the development of the cerebellum. 2) Deletion of CXCR4 in cerebellum causes premature migration of a population of GNPs throughout development that are capable of proliferation outside of their normal mitogenic niche. 3) Loss of CXCR4 causes a reduction in the activation of Sonic hedgehog pathway, but exerts normal proliferation in GNPs and MB.