一种多级孔生物活性玻璃的制备及固载溶菌酶研究

Hierarchically porous bioactive glass (noted as YMBG) was prepared by pine pollen for lysozyme immobilization via intermolecular interactions. The morphology and pore diameter distribution of YMBG were characterized by scanning electron microscopy (SEM), transmitted electron microscopy (TEM) and nitrogen adsorption- desorption. FTIR , UV, fluorescence, and circular dichroism (CD) studies were showed that lysozyme maintained its original secondary structure in the process of lysozyme immobilization. And the optimal experimental conditions were explored. This material possessed a high lysozyme immobilized amount (648±5.1 mg/g support). In addition, it was found that the adsorption process was suitable for the Langmuir isothermal model. And the activity of immobilized lysozyme still kept about 52% of the original enzyme; the use of immobilized enzyme was distinguished, and the activity remained about 25% after using 4 times repeatedly.