基于量子点标记的新型核酸探针的原位杂交方法

In situ hybridization is the short name of in situ hybridization histochemistry, and the probe preparation is a key factor for its detection. Quantum dots (QDs), also known as semiconductor nanocrystals, can activated by optical excitation or voltage, and has a broad emission spectrum. Compared with traditional organic dyes or fluorescent substance, QDs have more advantages such as more stable fluorescence, higher sensitivity, longer fluorescence time and better biocompatibility, et al. Thus, QDs have been dramatically used in biology and related applications. In this study, a new type of nucleic acid probe labeled with ZnS QD has been synthesized for the detection of GAPDH gene in longissimus dorsi muscle in Yorkshire pigs. The results show that QD-labeled probe has distinct positive signals, which indicated QDs can successfully detect glyceraldehyde-3-phosphate dehydrogenase gene (GAPDH) in pig muscles. These results provided an experimental foundation for the in situ hybridization based on QD-labeled probes and also shed light on the biomaterial applications of QDs-labeled probes for fluorescent detection in variable tissues.