苯甲酰胺抗炎活性研究

Inflammation is essential for regeneration and repair after tissue injury, but excessive inflammatory response or persistent inflammatory state can affect the state of tissue repair and even lead to tissue repair failure. Macrophages are one of the major effector cells of the innate immune response, which can be polarized into pro-inflammatory (M1) and anti-inflammatory (M2) types and are involved in the tissue repair process. Modulation of macrophage polarization has great potential to promote tissue repair. Benzamide (BEN), as an inhibitor of poly(adenosine diphosphate) ribose polymerase (PARP), an important inflammation-related regulator, may have anti-inflammatory active effects, but its effect on macrophage polarization has not been clarified. Therefore, in this paper, we assessed the effect of BEN on macrophage polarization in vitro to explore its role in the regulation of inflammation. An in vitro inflammation model was established using lipopolysaccharide-stimulated RAW264.7 cells, the cytotoxicity of BEN was detected by CCK-8, and macrophage pro-inflammatory and anti-inflammatory factor expression was detected by protein immunoblotting and immunofluorescence staining at the protein level, and the polarization status of macrophages was observed. The results showed that BEN inhibited the expression of macrophage pro-inflammatory factors, promoted the expression of anti-inflammatory factors, and regulated their M2-type polarization. The results of this study preliminarily suggest that BEN has anti-inflammatory activity, which lays the in vitro foundation for in vivo evaluation of its use in the treatment of inflammation-related diseases.