高分子量多环芳烃污染对包气带土壤中微生物群落的影响

Organic pollution in groundwater is getting worse. In China, polycyclic aromatic hydrocarbons (PAHs) are widespread in the groundwater, including high-molecular weight PAHs (HMW PAHs) like fluoranthene and pyrene. Soil samples, collected from vadose zone that has been polluted by crude oil for long time, was cultured in a mineral salt medium using fluoranthene and pyrene as the carbon and energy source for the selection of possible degrading bacteria. The microbial diversity and the relation of genus and species was analyzed adopting 16S rDNA sequencing method. Experimental results showed that the soil from vadose zone suffering from long-period crude oil pollution had a good microbial diversity. Besides, a bacterium belonging to an unknown genus was probably found. After incubation with fluoranthene and pyrene, the consortium in soils decreased to 4 species: Pseudomonas, Enterobacter, Hydrogenophaga and Luteolibacter pohnpeiensis. Results might provide scientifical basis for the further studies on the control and bioremediation of groundwater contamination.