茯苓多糖的制备、水解和生物活性研究
Preparation, hydrolysis and bioactivity evaluation of a natural polysaccharide from sclerotium of Poria cocos
从天然茯苓菌核中提取的茯苓多糖由于不溶于水使得其生物活性很低。为了提高茯苓多糖的生物活性，本研究通过酶催化的水解反应得到水溶性茯苓多糖。并且，为了进一步研究茯苓多糖的结构和生物活性之间的关系，制备了不同取代度的羧甲基化茯苓多糖和不同分子量的水溶性茯苓多糖。通过细胞实验评价了它们在S180肿瘤细胞中的抗肿瘤活性。我们的研究结果表明，酶催化水解和化学改性均能有效提高茯苓多糖的抗肿瘤活性。然而，我们的试验数据也提示在开发基于多糖的药物时，需要充分考虑多糖衍生物的分子量和取代度对其生物活性的影响。
医药卫生理论生物科学研究方法、生物科学研究技术药学
茯苓多糖抗肿瘤活性生物活性
杨佳,徐文瑨,陈琦.茯苓多糖的制备、水解和生物活性研究[EB/OL].(2016-03-15)[2025-11-02].http://www.paper.edu.cn/releasepaper/content/201603-194.点此复制
Pachyman isolated from a natural polysaccharide is lack of bioactivity due to its water-insolubility. In this study, in order to improve its bioactivity, pachyman was enzymatically hydrolyzed to obtain water soluble pachyman (WSP). Moreover, in order to further evaluate the relationship between the structure and bioactivity of pachyman, carboxymethylated pachyman (CMP) with different degree of substitution (DS) and water-soluble pachyman with different molecular weight were also prepared and their antitumor activities against S180 cells were further evaluated. Our results showed that both enzymatic hydrolysis and chemical modification were effective for improving the antitumor activity of pachyman. However, our data also suggested that the molecular weight and the degree of substitution of the derivatives should be taken into account when developing polysaccharide-based drugs.
Pachymanantitumor activitiesbioactivity
展开英文信息
评论