精神分裂症患者血清低表达量miRNA的检测
etection of microRNA with low abundance in serum of schizophrenia patients
最新研究表明血清中异常表达的microRNA可以反映疾病状态。然而，迄今为止反映精神分裂症疾病状态的血清microRNA相关研究尚未见报道。据文献报道，miR-346是最有可能反映精神分裂症的microRNA，本文研究发现半巢式定量PCR可以检测精神分裂症患者血清中低表达量的microRNA表达水平。而且，精神分裂症患者血清中miR-346表达水平明显比正常人血清中miR-346的表达水平高。家族史精神分裂症患者血清中miR-346表达水平明显比非家族史精神分裂症患者血清中miR-346表达水平低，这表明血清中miR-346表达水平与病人是否具有家族史有关。
Recent studies have revealed that aberrant expression of serum miRNA can directly reflect disease status. However, the study of serum miRNA for schizophrenia has not been reported so far. In this paper, the expression levels of serum miR-346 which is the most promising miRNAs to reflect schizophrenia status are studied. Semi-nested qRT-PCR was developed to detect miR-346 with very low abundance in serum sensitively and specifically. The results showed that the expression levels of miR-346 were significantly higher in schizophrenia patients than those in normal subjects. Compared with family schizophrenia patients, circulating miR-346 was significantly higher in sporadic schizophrenia, indicating that there is a positive correlation of circulating miRNA-346 level between schizophrenia patients with and without family history.
肖忠党、汪天宇、戚宇华、李树春、杨柯、梁高峰、史文婷、杜经纶、谢世平
神经病学、精神病学基础医学生物科学研究方法、生物科学研究技术
精神分裂症miR-346血清半巢式实时定量PCR
schizophreniamiR-346serumsemi-nested qRT-PCR
肖忠党,汪天宇,戚宇华,李树春,杨柯,梁高峰,史文婷,杜经纶,谢世平.精神分裂症患者血清低表达量miRNA的检测[EB/OL].(2010-12-02)[2025-08-03].http://www.paper.edu.cn/releasepaper/content/201012-35.点此复制
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