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Validating a screening agar for linezolid-resistant enterococci

Validating a screening agar for linezolid-resistant enterococci

来源:bioRxiv_logobioRxiv
英文摘要

Abstract Linezolid is an alternative treatment option for infections with multidrug-resistant Gram-positive bacteria including vancomycin-resistant enterococci (VRE). Some countries report an increasing number of isolates with resistance to linezolid. The recent publication of the Commission for Hospital Hygiene in Germany on enterococci/VRE recommends screening for linezolid-resistant enterococci (LRE). However, a suitable selective medium or a genetic test is not available. Our aim was to establish a selective screening agar for LRE detection and validate its application with a comprehensive collection of clinical LRE and linezolid-susceptible enterococci (LSE). We decided to combine the selective power of an enterococcal screening agar with a supplementation of linezolid. Several rounds of analyses with reference, control and test strains pointed towards Enterococcosel agar and a concentration of 2 mg/L linezolid. Finally, we validated our LRE agar with 400 samples sent to our National Reference Centre in 2019.

Werner Guido、Klare Ingo、Weber Robert E.、Fleige Carola、Bender Jennifer K.

10.1101/798983

医学研究方法微生物学药学

cfroptrApoxtAlinezolid resistance

Werner Guido,Klare Ingo,Weber Robert E.,Fleige Carola,Bender Jennifer K..Validating a screening agar for linezolid-resistant enterococci[EB/OL].(2025-03-28)[2025-08-25].https://www.biorxiv.org/content/10.1101/798983.点此复制

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