Validating a screening agar for linezolid-resistant enterococci
Validating a screening agar for linezolid-resistant enterococci
Abstract Linezolid is an alternative treatment option for infections with multidrug-resistant Gram-positive bacteria including vancomycin-resistant enterococci (VRE). Some countries report an increasing number of isolates with resistance to linezolid. The recent publication of the Commission for Hospital Hygiene in Germany on enterococci/VRE recommends screening for linezolid-resistant enterococci (LRE). However, a suitable selective medium or a genetic test is not available. Our aim was to establish a selective screening agar for LRE detection and validate its application with a comprehensive collection of clinical LRE and linezolid-susceptible enterococci (LSE). We decided to combine the selective power of an enterococcal screening agar with a supplementation of linezolid. Several rounds of analyses with reference, control and test strains pointed towards Enterococcosel agar and a concentration of 2 mg/L linezolid. Finally, we validated our LRE agar with 400 samples sent to our National Reference Centre in 2019.
Werner Guido、Klare Ingo、Weber Robert E.、Fleige Carola、Bender Jennifer K.
医学研究方法微生物学药学
cfroptrApoxtAlinezolid resistance
Werner Guido,Klare Ingo,Weber Robert E.,Fleige Carola,Bender Jennifer K..Validating a screening agar for linezolid-resistant enterococci[EB/OL].(2025-03-28)[2025-08-25].https://www.biorxiv.org/content/10.1101/798983.点此复制
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