香鱼巨噬细胞对鳗弧菌的吞噬作用

yu, Plecoglossus altivelis, is a popular and highly valued edible fish. We established primary culture of head kidney-derived macrophages of ayu. Moreover, the phagocytic capability and respiratory burst response of macrophages were observed when these cells were treated with Listonella anguillarum. The macrophages were obtained from head kidney of ayu by Ficoll gradient centrifugation, and removing non-adherent cells. The Giemsa staining was employed to confirm the morphology of ayu macrophages. The respiratory burst was estimated by measuring the intracellular O2- using the NBT reduction test. The results showed that the capability of ayu macrophages to generate a respiratory burst upon stimulation of Listonella anguillarum was higher than control. After adding Listonella anguillarum to cell media, an initial increase in the number of bacteria in the macrophages was seen at 4 h post-infection; a pronounced decrease was seen at 12 h post-infection. Our results suggest that ayu macrophages have the capability to kill Listonella anguillarum, which is an important host defense mechanism for Listonella anguillarum infected ayu.