菜豆蛋白磷酸酶PvPS2:1的表达模式及其功能研究

Protein dephosphorylation mediated by protein phosphatases plays a major role in signal transduction of plant responses to environmental stresses. In the study, one putative protein phosphatases, PvPS2:1 was characterized in bean. PvPS2:1 was found to be localized to the plasma membrane and the nucleus by transient expression of PvPS2:1:GFP in onion (Allium cepa) epidermal cells. Transcripts of PvPS2:1 were significantly increased by phosphate (Pi) starvation in the two bean genotypes, G19833 (a P-efficient genotype) and DOR364 (a P-inefficient genotype). However, G19833 exhibited higher PvPS2:1 expression levels than DOR364 in both leaves and roots during Pi starvation. Analysis of PvPS2:1 overexpression lines in bean hairy roots and Arabidopsis plants showed that PvS2:1 was involved in root growth and P accumulation. Furthermore, expression levels of several Pi starvation responsive genes were up-regulated in the overexpressing PvPS2:1 Arabidopsis. Taken together, our results strongly suggested that PvPS2:1 might be involved in Pi acquisition, and could be targeted as candidate gene to improve P efficiency in crops.