吡格列酮对糖尿病大鼠冠状动脉

IObjectiveTo observe RhoA and ROCK protein express level in diabetes mellitus rat's coronary artery tissue and identify whether pioglitazone could affect RhoA and ROCK protein expression level in diabetes mellitus Rat's cornary artery . Methods: 30 male Sprague-Dawley (SD) Rats was randomly divided into 3 groups,10 in each group.The control group was feed with normal diet ,10 in diabetes mellitus group (creat model by high fructose diet plus Streptozotocin（25mg/Kg） injection ),10 in pioglitazone group (DM plus pioglitazone10mg/kg/d).After 4 weeks treatment,blood samples were collected for glucose, serum lipid and serum insulin test. Coronary arteries were isolated for Westen blot assay to identify RhoA,ROCK1,ROCK2 proten expression level.Results : Compared with DM group, pioglitazone can observably lower cholesterol，triglyceride，fasting insulin level（P＜0.05）.The RhoA,ROCK2 protein express level were u-p regulated in DM group than control group,but ROCK1 proten express level was no change.The ROCK2 protein express level remarkably down regulated in PIO group（P=0.045），RhoA protein express level was lower than DM group ,but the difference was not reach statistics significance. Conclusions: Pioglitazone can remarkably reverse DM rat's insulin resistence,lower TC and TG level.The Rho/ROCK protein express is increased in the DM rat's coronary artery, Pioglitazone can significantly down regulated ROCK2 protein express level in the DM rat's coronary artery, RhoA protein express level also has a lower tendency，eventhough couldn't achieve statistics significance.