Klebsiella pneumoniae yqhD的克隆与表征
loning and Characterization of yqhD from Klebsiella pneumoniae
本文克隆了Klebsiella pneumoniae DSM2026的醛还原酶基因yqhD并在E. coli BL21(DE3)中得到了高效表达，经过Q-Sepharose和Sephacryl S-300两步柱层析纯化得到电泳条带单一的纯酶。活性检测表明该酶为醛还原酶，催化3-羟基丙醛等多种底物还原生成相应的醇。以3-羟基丙醛为底物，该酶的最适pH为5.8，最适温度为60 ℃，对NADPH和NADH的Km值分别为0.07 mM和1.42 mM。
In this paper Klebsiella pneumoniae yqhD was cloned and overexpressed in E. coli BL21(DE3). The enzyme was purified to homogeneity by Q-Sepharose and Sephacryl S-300. The catalytic characteristics of the enzyme were studied and it was an aldehyde reductase. Using 3-hydroxyaldehyde as substrate, the optimal pH and optimal temperature of the enzyme were pH 5.8 and 60 ℃, respectively, and the Km values for NADPH and NADH were 0.07 mM and 1.42 mM, respectively, which demonstrated the flexibility of dependence on coenzymes for this enzyme.
修志龙、张乐、戴建英、马成伟
生物化学分子生物学微生物学
Klebsiella pneumoniaeyqhD3-羟基丙醛13-丙二醇
Klebsiella pneumoniaeyqhD3-hydroxyaldehyde13-propanediol
修志龙,张乐,戴建英,马成伟.Klebsiella pneumoniae yqhD的克隆与表征[EB/OL].(2009-09-04)[2025-08-02].http://www.paper.edu.cn/releasepaper/content/200909-158.点此复制
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