Size control in mammalian cells involves modulation of both growth rate and cell cycle duration
Size control in mammalian cells involves modulation of both growth rate and cell cycle duration
Summary Despite decades of research, it remains unclear how mammalian cell growth varies with cell size and across the cell division cycle to maintain size control. Answers have been limited by the difficulty of directly measuring growth at the single cell level. Here we report direct measurement of single cell volumes over complete cell division cycles. The volume added across the cell cycle was independent of cell birth size, a size homeostasis behavior called “adder”. Single-cell growth curves revealed that the homeostatic behavior relied on adaptation of G1 duration as well as growth rate modulations. We developed a general mathematical framework that characterizes size homeostasis behaviors. Applying it on datasets ranging from bacteria to mammalian cells revealed that a near-adder is the most common type of size control, but only mammalian cells achieve it using modulation of both cell growth rate and cell-cycle progression.
Monnier Sylvain、Attia Rafaele、Baum Buzz、Piel Matthieu、Grilli Jacopo、Terriac Emmanuel、Cadart Clotilde、Cosentino-Lagomarsino Marco
Institut Curie, PSL Research University||Univ. Lyon, Universit¨| Claude Bernard Lyon 1, CNRS, Institut Lumi¨¨re Mati¨¨reInstitut Curie, PSL Research University||Institut Pierre-Gilles de Gennes, PSL Research UniversityMRC Laboratory for Molecular Cell Biology||Institute of Physics of Living SystemsInstitut Curie, PSL Research University||Institut Pierre-Gilles de Gennes, PSL Research UniversityDepartment of Ecology and Evolution, University of ChicagoInstitut Curie, PSL Research University||INM-Leibniz Institute for New MaterialsInstitut Curie, PSL Research University||Institut Pierre-Gilles de Gennes, PSL Research UniversitySorbonne Universit¨|s, Universit¨| Pierre et Marie Curie||CNRS, UMR 7238 Computational and Quantitative Biology||FIRC Institute of Molecular Oncology (IFOM)
细胞生物学生物科学现状、生物科学发展生物科学研究方法、生物科学研究技术
Cell volumecell size homeostasiscell division cyclemammalian cellsgrowth ratemicrochannelsmicrofabricationlive cell microscopy
Monnier Sylvain,Attia Rafaele,Baum Buzz,Piel Matthieu,Grilli Jacopo,Terriac Emmanuel,Cadart Clotilde,Cosentino-Lagomarsino Marco.Size control in mammalian cells involves modulation of both growth rate and cell cycle duration[EB/OL].(2025-03-28)[2025-05-01].https://www.biorxiv.org/content/10.1101/152728.点此复制
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