基于DNA纳米结构的细胞膜囊泡靶向功能化修饰研究

Benefited by good biocompatibility and low immunogenicity, cell-derived nanovesicles have been used to deliver diagnostic and therapeutic agents. Although their intrinsic properties make cell-derived nanovesicles excellent nanocarriers for targeted delivery, the lack of active targeting capabilities of the vesicles may cause off-target effects. To solve this problem, we use DNA nanostructures containing nucleic acid aptamers to modify cell membrane vesicles. By constructing a linear strand hybridization reaction (HCR) DNA structure, and combining a nucleic acid aptamer (a single-stranded nucleic acid that can specifically bind to the DNA sequence of various targets from ions to proteins) in the repeating units of the HCR structure. And through the cholesterol group to modify the DNA structure and combine it with the cell membrane vesicles, thereby giving the cell membrane vesicles the ability to target and bind to target cells. This modification method based on DNA nanotechnology provides new ideas for the functionalization of cell membrane vesicles and further expands the biomedical applications of cell membrane vesicles.